中文摘要：

采用溶剂热法合成了共价有机框架材料,并使铂（Ⅳ）通过电化学还原沉积于此材料上,制成作为固定化基质的Pt/COF-LZUI。另据文献分别制备了MnO2纳米材料,Pd NPs/MnO2纳米复合材料,及以此为标记物的CRP抗体。用上述材料按规定程序修饰玻碳电极并制成夹心型CRP免疫传感器。利用固体核磁、X射线粉末衍射对COF-LZUI的结构和晶型结构进行了表征,利用透射电镜对COF-LZUl、Pt/COF-LZUl和Pd NPs/MnO2纳米复合材料的形貌进行了表征。采用循环伏安法和计时电流法（i-t）研究该传感器的电化学特性及该电极对过氧化氢的电化学响应。该传感器的线性范围为1-150μg·L^-1,检出限（3σ）为0.33μg·L^-1。

英文摘要：

Covalent organic framework material （COF-LZUI） was synthesized by the solvent thermal method and Pt-metal was deposited on it by electrochemical reduction of Pt（ IV ） to give Pt/COF-LZUI, which was used as immobilization matrix for CRP. Nanoparticles of MnO2, composite nanoparticles of Pd NPs/MnO2 and Pd NPs/ MnO2 marked CRP were prepared separately as described in literatures. The above mentioned materials were used to modify GCE giving the sandwich immunosensor for CRP as specified in the procedure of preparation. The structure and crystallization of COF-LZUI were characterized by MAS NMR and XRD, and the morphology of COF-LZU1, Pt/COF-LZU1, Pd NPs/MnO2 nano-composites were characterized by TEM. Its electrochemical behavior and response to H2O2 were studied by cyclic voltammetry and chronoamperometry （i-t）. Linearity range of the sensor found was 1-150μg·L^-1 and value of detection limit （3σ） was 0. 33μg·L^-1.