中文摘要：

从HearNPV G4株基因组中克隆得到orf80基因的全序列,将该基因构建于pMAL-c4E载体并在TB1中融合表达,orf80融合表达蛋白分子质量为73.3ku,与预测的蛋白一致,Western blot验证正确。纯化融合蛋白后制备抗原并注射新西兰大白兔从而得到orf80的多克隆抗体,经ELISA检测其效价达到2.56×105,表明该抗体具有较高的免疫原性,可用于orf80编码蛋白的检测及蛋白互作等相关研究。

英文摘要：

Abstract： The HearNPV orf80 was cloned from Helicoverpa armigera Single-nucleopolyhedrovirus （HearNPV） G4 genome by PCR and the prokaryotic expression vector pMAL-c4E-Ha80 was con- structed. The recombinant plasmid was transformed into the Escherichi coli TB1 to express the MBP- HaS0 fushion protein. Purified MBP-Ha80 protein was injected into rabbits as antigen to prepare the polyclonal antibodies of orf80. The titer of anti-HearNPV off80 polyclonal antibodies was 2.56 × 10^5 detected by ELISA. The prepared antibodies can be used to determine orf80 coding protein in the re- search of protein detection and protein interaction.