在 Spodoptera litura 赘言作家的 midugt 积累的镍能导致 metallothionein 的表示,在有机体的最重要的解毒作用蛋白质之一。在现在的学习,在解毒作用酶的活动的饮食的镍的效果,例如 carboxylesterase (照顾) 和在 S 的中间的内脏的谷胱甘肽 S-transferase (GST ) 。litura 幼虫被学习了得到 S 的解毒作用机制的理解。litura 幼虫到过多的镍。结果证明在第 5 中间形态幼虫的中间的内脏的照顾活动在镍(⩽5 mg/kg ) 的底层减少了,当与在镍(⩾10 mg/kg ) 的高水平增加镍剂量增加了时暴露在连续 3 代。第 6 中间形态幼虫的照顾活动是在镍暴露的底层禁止了也被描绘,并且在第一产生在高水平改善了。在第三产生都是的第二 and 的第 6 中间形态幼虫的照顾活动在控制比那降低。然而,在第 5 和第 6 中间形态幼虫的中间的内脏的 GST 活动都与在食谱增加镍剂量(1 20 mg/kg ) 增加了。
Nickel accumulated in midugt of Spodoptera litura Fabricius could induce the expression of metallothionein, one of the most important detoxification proteins in organisms. In the present study, the effects of dietary nickel on the activities of detoxification enzymes, such as carboxylesterase (CarE) and giutathione S-transferase (GST) in the midgut of S. litura larvae have been studied to get an understanding of the detoxification mechanisms of S. litura larvae to excessive nickel. Results showed that CarE activities in the midgut of the 5th instar larvae decreased at lower levels of nickel (≤5 mg/kg), while increased with increasing nickel doses at higher levels of nickel (≥10 mg/kg) exposure in successive 3 generations. CarE activities of the 6th instar larvae were also characterized as inhibited at low levels of nickel exposure, and improved at higher levels in the 1st generation. CarE activities of 6th instar larvae in the 2nd and 3rd generations were all lower than that in control. However, GST activities in the midgut of the 5th and 6th instar larvae all increased with increasing nickel doses (1 --20 mg/kg) in diets.