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Effects of dietary nickel on detoxification enzyme activities in the midgut of Spodoptera litura Fabricius larvae
  • ISSN号:0454-6296
  • 期刊名称:《昆虫学报》
  • 时间:0
  • 分类:R574[医药卫生—消化系统;医药卫生—临床医学;医药卫生—内科学]
  • 作者机构:[1]State key laboratory for Biological Control & Institute of Entomology, Sun Yat-sen University, Guangzhou 510275, China
  • 相关基金:Supported by the National Basic Research Development Program of China (Grant No. 2006CB102001), National Natural Science Foundation of China (Grant No. 30771458), Doctoral Fundaytion of Ministry of Education of China (Grant No. 20070558029) and Basic Research Group Programs of Guangdong Province (Grant No. E039254)
中文摘要:

在 Spodoptera litura 赘言作家的 midugt 积累的镍能导致 metallothionein 的表示,在有机体的最重要的解毒作用蛋白质之一。在现在的学习,在解毒作用酶的活动的饮食的镍的效果,例如 carboxylesterase (照顾) 和在 S 的中间的内脏的谷胱甘肽 S-transferase (GST ) 。litura 幼虫被学习了得到 S 的解毒作用机制的理解。litura 幼虫到过多的镍。结果证明在第 5 中间形态幼虫的中间的内脏的照顾活动在镍(⩽5 mg/kg ) 的底层减少了,当与在镍(⩾10 mg/kg ) 的高水平增加镍剂量增加了时暴露在连续 3 代。第 6 中间形态幼虫的照顾活动是在镍暴露的底层禁止了也被描绘,并且在第一产生在高水平改善了。在第三产生都是的第二 and 的第 6 中间形态幼虫的照顾活动在控制比那降低。然而,在第 5 和第 6 中间形态幼虫的中间的内脏的 GST 活动都与在食谱增加镍剂量(1 20 mg/kg ) 增加了。

英文摘要:

Nickel accumulated in midugt of Spodoptera litura Fabricius could induce the expression of metallothionein, one of the most important detoxification proteins in organisms. In the present study, the effects of dietary nickel on the activities of detoxification enzymes, such as carboxylesterase (CarE) and giutathione S-transferase (GST) in the midgut of S. litura larvae have been studied to get an understanding of the detoxification mechanisms of S. litura larvae to excessive nickel. Results showed that CarE activities in the midgut of the 5th instar larvae decreased at lower levels of nickel (≤5 mg/kg), while increased with increasing nickel doses at higher levels of nickel (≥10 mg/kg) exposure in successive 3 generations. CarE activities of the 6th instar larvae were also characterized as inhibited at low levels of nickel exposure, and improved at higher levels in the 1st generation. CarE activities of 6th instar larvae in the 2nd and 3rd generations were all lower than that in control. However, GST activities in the midgut of the 5th and 6th instar larvae all increased with increasing nickel doses (1 --20 mg/kg) in diets.

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期刊信息
  • 《昆虫学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国昆虫学会 中国科学院动物研究所
  • 主编:黄大卫
  • 地址:北京东中关村中国科学院动物研究所
  • 邮编:100101
  • 邮箱:kcxb@ioz.ac.cn
  • 电话:010-64807173 010-64807099
  • 国际标准刊号:ISSN:0454-6296
  • 国内统一刊号:ISSN:11-1832/Q
  • 邮发代号:2-153
  • 获奖情况:
  • 国家期刊方阵“双百期刊”
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:16190