目的 明确永生化人成牙本质细胞样细胞系hTERT-hOd-1的生物学特征。方法在连续体外培养过程中,观察细胞的形态学特征和生长增殖特征并与人成牙本质细胞样细胞比较。结果细胞形态为成纤维细胞样,细胞器发达,生长增殖活跃,具有克隆形成能力。结论hTERT-hOd-1保持了人成牙本质细胞样细胞的生物学特征。
Objective To explicate the biological characters of an immortal human odontoblast-like cell line hTERT-hod-1. Methods The morphology, growth and proliferation of the cells were observed during continuous culture in vitro and compared with that of human odontoblast-like cells, Results The hTERT-hod-1 cells showed fibroblast-like feature with rich in organelle. They grew and proliferated well and were able to form cell clone in vitro . Conclusion The hTERT-hOd-1 remains the biological characters of human odontoblast-like cells.