中文摘要：

目的研究热化联合对肺部肿瘤生长的影响及其可能的机制。方法采用临床常用剂量（43℃加热联合50μg/L紫杉醇）,通过单纯化疗、单纯热疗及热化联合的方法处理肺癌A549细胞,以未处理的A549细胞作对照,应用噻唑蓝比色法检测各处理方式下细胞增殖率的变化,进行损伤修复实验观察比较各组细胞的侵袭力,并通过蛋白免疫印记法检测JNK磷酸化和热休克蛋白70（HSP70）的表达。结果热化联合组细胞增殖率低于单纯化疗、单纯热疗及热化联合加JNK抑制剂SP600125组（P〈0.05）,而热化联合加SP600125组的细胞增殖率与对照组相比,差异无统计学意义（P〉0.05）;热化联合组的细胞侵袭力较其他组有所减弱;热化联合组JNK磷酸化的水平高于对照组及单纯化疗组（P〈0.05）;HSP70在热化联合组的表达低于单纯热疗组（P〈0.05）。结论热化联合对肺癌A549细胞生长增殖的抑制作用强于单纯热疗和单纯化疗,这种抑制作用可能是通过激活JNK信号通路或抑制HSP70的表达完成的。

英文摘要：

Objective To study the synergistic effect of thermotherapy and chemotherapy with chemotherapy on lung tumor cell growth in order to explore its underlying mechanisms.Methods A549 cells were treated by combination of 43℃ and 50μg/L Paclitaxel,and 43℃ or 50μg/L Paclitaxel alone.MTT assay and Wound-healing assay were used to measure the survival and the invasive capacity.Phosphorylation of JNK and expression of HSP70 were determined with Western Blotting.Cells without treatment were used as controls.Results In comparison with cells treated with 43℃ and 50μg/L Paclitaxel alone,or with combination of 43℃ and 50μg/L Paclitaxel and SP600125,the proliferation rate of cells subjected to combination treatment of 43℃ and 50μg/L Paclitaxel decreased significantly（P〉0.05）.There were no significant differences in cell proliferation between untreated cells and the cells treated with 43℃ heat,50μg/L Paclitaxel and SP600125（P〉0.05）.The invasive capacities of the cells treated combination of 43℃ heat and 50μg/L Paclitaxel were markedly reduced in comparison to other treatment groups.Also phosphorylations of JNK were significantly elevated in comparison to untreated cells or the cells only underwent chemotherapy（P〈0.05）.The expression levels of HSP70 on thermotherapy in combination with chemotherapy were more lower than those in thermotherapy group（P〈0.05）.Conclusion Thermotherapy in combination with chemotherapy showed more strong inhibitory effect than those of thermotherapy or chemotherapy alone on lung tumor cell growth.The resultant phosphorylation of JNK and inhibition of HSP70 expression could be responsible for this effect.