中文摘要：

【目的】筛选5龄飞蝗不同发育时间的最适内参基因，为相关研究提供基础数据。【方法】本文选取β-肌动蛋白（β-actin）、延长因子（EF-1α）、3．磷酸甘油醛脱氢酶（GAPDH）、核糖体蛋白49（RP49）、α-微管蛋白（α-Tubulin）和18S核糖体RNA（18SrRNA）基因作为候选内参基因，运用实时定量PCR（qPCR）方法研究各基因在5龄飞蝗不同发育时间的相对表达量，用geNorm与Normfinder软件分析这6个基因表达稳定性。【结果】geNorrn分析结果显示6个内参基因表达稳定度M值顺序为：β-actin（0．3720）〉RP49（0．3750）〉α-Tubulin（0．4030）〉18S rRNA（0．4270）〉EF-15（0．4970）〉GAPDH（0．6040）。M值越小表示基因表达稳定度越高，同时geNorm软件以标准化因子配对差异值（Pairwisevariations）0．15默认为取舍值，由于V2／3=0．098〈0．15，所以最适内参基因数目为2个。运用NormFinder软件也得出相似的结果。【结论】β-actin与RP49为5龄飞蝗不同发育时间的最适内参基因。

英文摘要：

[Objectives] To identify the most appropriate reference genes on different days during the development of fifth-instar nymphs of Locusta migratoria to pave the way for relevant studies. [Metllods] Six genes were selected as candidate reference genes, including the beta actin gene （β-actin）, the Elongation factor la gene （EF-la）, the Glyeeraldehyde-3-phosphate dehydrogenase gene （GAPDH）, the Ribosomal protein 49 gene （RP49）, the Alpha tubulin gene （α-Tubulin） and the 18S ribosome RNA gene （18S rRNA）. The relative expression of these genes on different days during the development of L. migratoria was detected with quantitative real-time PCR （qPCR）. The expression stability of these genes was then investigated using geNorm and Normfinder software. [Results] Based on the results of geNorm analysis, the average expression stability （M） of the six genes was, in descending order; fl-actin （0.3720） 〉 RP49 （0.3750） 〉 α-Tubulin （0.4030） 〉 18S rRNA （0.4270） 〉 EF-la （0.4970） 〉 GAPDH （0.6040）. The less the M value the more stable the gene expression. The software took 0.15 as a constant value for pairwise variations. The appropriate number of reference genes was 2 because V2/3=0.098 〈 0.15. Similar results were obtained using NormFinder software. [Conclusion] β-actin and RP49 are the most suitable reference genes during the development of fifth-instar nymph ofL. migratoria.