中文摘要：

目的：探讨HIF-1α、MT、Slug和E-cadherin在人乳腺癌中的表达及其意义。方法：采用免疫组化SP法,检测术前未使用放疗、化疗及激素治疗的69例乳腺癌组织中HIF-1α、MT、Slug和E-cadherin的表达情况。采用RT-PCR方法检测乳腺癌细胞系MCF-7和MDA-MB-231中HIF-1α、MT、Slug和E-cadherin mRNA的表达情况。结果：免疫组化结果表明：在69例乳腺癌组织中,HIF-1α、MT、Slug和E-cadherin的阳性率分别为71.01%、42.03%、43.48%和50.72%。HIF-1α、Slug和E-cad-herin的表达对于TNM分期和腋淋巴结转移有显著性差异（P〈0.05）;MT的表达对于TNM分期有显著性差异（P〈0.05）。在69例乳腺癌组织标本中HIF-1α与MT表达呈显著正相关（r=0.285,P〈0.05）,MT与Slug呈显著正相关（r=0.379,P〈0.01）,Slug与E-cadherin呈显著负相关（r=-0.422,P〈0.01）。RT-PCR半定量结果显示：HIF-1α、MT、Slug和E-cadherinmRNA在人乳腺癌细胞系MCF-7和MDA-MB-231中均有表达,但在恶性程度更高的MDA-MB-231细胞中HIF-1α、MT、Slug表达较高,而E-cadherin表达较低,差异具有显著性（P〈0.05）。结论：HIF-1α、MT、Slug和E-cadherin在乳腺癌中的表达情况可作为监测乳腺癌发展的指标。

英文摘要：

Objective：To investigate the expressions and significances of HIF- 1α, MT, Slug and E-cadherin in human breast cancer. Methods： Immunohistochemistry was used to analyze the expression of HIF-1α, MT, Slug and E-cadherin in 69 tissue samples of breast cancer patients without any radiotherapy, chemotherapy and hormone therapy. The mRNA expressions of HIF-1 α, MT, Slug and E-cadherin in breast cancer cell lines MCF-7 and MDA-MB-231 were detected by RT-PCR analysis. Results：In 69 tissue samples of breast cancer, the positive expression rates of HIF-1α, MT, Slug and E-cadherin were 71.01%, 42.03% , 43.48% and 50.72%. The expressions of HIF-1α, Slug and E-cadherin have significant correlations with TNM stage and axillary lymph node metastasis （P 〈 0.05 ）, the expression of MT has a significant correlation with TNM stage （ P 〈 0.05 ）. In 69 tissue samples of breast cancer, the expression of HIF-1 ct has a positive correlation with that of MT （ r = 0. 285, P 〈 0.05 ） , the expressions of MT and Slug were significantly positively correlated （r = 0. 379, P 〈 0.01 ）, and the expression of Slug was significantly negatively correlated with that of E-cadherin （r = -0.422 ,P 〈 0. 01 ） in breast cancer. RT-PCR analysis showed that HIF-1α, MT, Slug and E-cadherin mRNA were expressed in breast cancer cell lines MCF-7 and MDA-MB-231. The mRNA expression levels of HIF-1α, MT and Slug was higher and the expression levels of E-cadherin was lower in MDA-MB-231 cell line than in MCF-7 cell line （ P 〈 0.05 ）. Conclusion ： The expressions of HIF-1α, MT, Slug and E-cadherin might be used as markers for development of breast cancer.