中文摘要：

目的探讨低氧诱导因子-1（HIF-1α）、Smad蛋白的相互作用蛋白1（SIP1）和基质金属蛋白酶-9（MMP-9）在甲状腺乳头状癌（PTC）中的表达及意义。方法采用免疫组化SP法检测72例人甲状腺乳头状癌和37例癌旁正常组织中HIF-1α、SIP1和MMP-9的表达水平,分析其表达与临床病理指标的关系及三者间表达的相关性。结果在PTC组织中,HIF-1α、SIP1和MMP-9的阳性表达率分别为51.56%、51.39%和54.16%,显著高于癌旁正常组织（P〈0.01）。HIF-1α、SIP1和MMP-9的表达与患者颈部淋巴结转移显著相关（P〈0.05）。HIF-1α与SIP1、HIF-1α与MMP-9、SIP1和MMP-9的表达均呈显著正相关（rs=0.304,P=0.009;rs=0.243,P=0.040;rs=0.277,P=0.019）。2种蛋白（HIF-1α/SIP1,HIF-1α/MMP-9,SIP1/MMP-9）联合表达较二者之一表达与颈部淋巴结转移更具相关性（P〈0.05）。HIF-1α、SIP1和MMP-9 3种蛋白联合表达较三者之一表达与颈部淋巴结转移更具有相关性（P〈0.05）。结论 HIF-1α、SIP1和MMP-9在PTC组织中的表达与颈部淋巴结转移密切相关,且三者存在相互协同作用,其表达情况可能作为监测PTC颈部淋巴结转移的指标。

英文摘要：

To investigate the expression and significance of HIF-1α,SIP1 and MMP-9 in papillary thyroid carcinoma（PTC）,we analyzed the expressions of HIF-1α,SIP1 and MMP-9 in 72 PTC samples by immunohistochemistry.Furthermore,the correlations of HIF-1α,SIP1 and MMP-9 expression with one another,and with several clinicopathological indicators were statistically analyzed.In PTC samples,the positive expression rates of HIF-1α,SIP1 and MMP-9 were 51.56%,51.39% and 54.16% respectively,which were significantly higher than those in the normal thyroid group（P 0.01）;HIF-1α,SIP1 and MMP-9 expression had significant correlations with cervical lymph node metastasis（P〈 0.05）.Meanwhile,HIF-1α expression had a positive correlation with SIP1（rs=0.304,P=0.009） and MMP-9（rs=0.243,P=0.040）,wihle SIP1 and MMP-9 expression were positively correlated（rs=0.277,P=0.019）.Concomitant expression of any two or all of the three molecules had stronger correlation with lymph node metastasis than did each alone（P〈 0.05）.In conclusion,HIF-1α,SIP1 and MMP-9 in PTC samples work synergistically and closely correlated to cervical lymph node metastasis.Therefore,the expression of HIF-1α,SIP1 and MMP-9 might be used as biomarkers for cervical lymph node metastasis of PTC.