中文摘要：

【目的】从发生番茄青枯病的土壤微生物群落Biolog测定结果中，在ECO微平板10种特征碳源基础上，减为9种特征碳源，利用MT板进行试验，以简化其测定手续。【方法】选用盆栽番茄土样进行了特征碳源的浓度试验，并应用所确定的浓度分别针对施肥处理不同但均未发病的大田辣椒土样、发病程度不同的大田番茄土样和盆栽辣椒土样进行了3次验证试验。【结果】浓度试验结果表明，各碳源不同浓度的0D值普遍存在差异，将其与ECO板结果相关联后确定了各特征碳源的应用浓度。3次验证试验结果表明，选用的试验浓度能明显地区分作物发病差异。【结论】特征碳源能反映出茄科作物病土与健康土以及发病轻与重的区别，可以简化土壤微生物Biolog测定。

英文摘要：

[Objective] In order to simplify the Biolog analysis, 9 kinds of characteristic carbon sources selected from the carbon resources in Biolog ECO microplate containing 10 carbon resources and added in Biolog MT microplate to analyse soil microbial cummunity where bacterial wilt of tomato occurred. [Method] The optimal concentration of characteristic carbon resources added in Biolog MT microplates was studied with the soil of potted tomato and three tests of verifying the carbon resource concentrations were carried out by using the soils of potted pepper without soil-borne diseases occurrence treated with different fertilization rates and the soils of field tomato and potted pepper with varied incidences of bacterial wilt. [Result] The result of carbon resource concentration experiment showed that the OD values of different concentrations of characteristic carbon resources were obviously different. Compared with the OD in ECO microplate, the optimal concentrations in MT microplate were determined. The results of verifying tests indicated that the soil microbial community functional diversity （Shannon index） with different incidences of crop were differentiated by characteristic carbon resources. [Conclusion] Therefore, the characteristic carbon resources could be used to differentiate the health soil and disease soil, and the soils with varied incidences of soil-borne diseases. The Biolog analysis can be simplified by using 9 kinds of characteristic carbon resources.