中文摘要：

目的：探讨乙醇诱导肝星状细胞（hepatic stellate cell，HSC）凋亡模型的作用及其与凋亡相关基因caspase-3、p53、bax和bcl-2表达的关系。方法：用CCK-8法检测不同浓度（0、10、20、40、60 和80 mL / L）的乙醇对HSC的杀伤作用。采用40 mL / L乙醇作用6 h诱导HSC细胞凋亡。通过流式细胞术分析乙醇诱导对凋亡的影响，并用实时定量PCR检测乙醇诱导对凋亡相关基因caspase-3、p53、bax和bcl-2表达的影响。结果：随着乙醇浓度的增加，对HSC的杀伤能力逐渐增加。40 mL/ L乙醇使HSC发生显著的细胞凋亡变化。流式细胞术检测发现乙醇诱导可使HSC早期＋晚期凋亡明显增加（8.76% ± 0.43% vs 11.51% ± 0.54%），差异具有统计学意义（t = 2.11，P 〈 0.05）；促凋亡基因caspas-3、bax和p53的mRNA表达水平上调，抑制凋亡基因bcl-2的mRNA 表达水平下调。结论：低浓度乙醇可诱导HSC凋亡增加，其凋亡发生与凋亡相关基因caspase-3、p53、bax和bcl-2表达改变相关。

英文摘要：

Objective： To establish ethanol-induced apoptosis in hepatic stellate cell（HSC） and analyze the expression of apoptosis-related genes, caspase-3, p53, bax and bcl-2. Methods： HSC was exposed to different concentrations of ethanol（0、10、20、40、60 and 80 mL / L） for 6 h, and the cytotoxic effect was analyzed by CCK8 assay. Then HSC was treated with 40 mL/L of ethanol for 6 h, apoptosis was analyzed by FACS, and expression of apoptosis-related genes （caspase-3, p53, bax and bcl-2） was evaluated by real-time PCR. Results： The cytotoxic effect of ethanol is directly correlated with its concentration. At 40 mL/L, ethanol induced significant apoptosis in HSC as evaluated by FACS, and significantly increased the expression of caspase-3, p53, and bax, and significantly decreased the expression of bcl-2 as evaluated by real-time PCR. Conclusion： Low dose of ethanol can induce apoptosis in HSC, which may be associated with the altered expression of caspase-3, p53, bax and bcl-2.