以上海老港垃圾填埋场配套污水处理设施中的污泥为菌种源。在序批式活性污泥反应器(SBR)中对晚期垃圾渗滤液进行短程硝化处理,调节SBR中溶解氧浓度,考察溶解氧对渗滤液短程硝化的影响,分析不同溶解氧条件下污泥微生物群落结构的变化。结果表明,低溶解氧(0.2~0.5mg/L)条件下,SBR可以获得较高的短程硝化效率,反应17h后,SBR内亚硝态氮/氨氮(质量比)为1.05,氨氮负荷可达到1.5kg/(kg·d)(以每千克污泥悬浮固体每天承担的氨氮计),出水可以满足后续厌氧氨氧化处理的要求。从污泥变形梯度凝胶电泳(DGGE)图谱中可以看出,SBR微生物群落结构中主要优势种有uncultured Bacteroidetes bacterium、uncultured bacterium、uncultured Candidatus Amoebophilus sp.等。随着溶解氧含量的升高,SBR内微生物群落结构的多样性有所升高,但溶解氧对微生物群落结构影响有限。
The active sludge collected from sewage treatment facilities of Shanghai Laogang municipal solid waste landfill was used as strain source for the shortcut nitrification treatment of aged landfill leachate in sequencing batch reactor (SBR). The effect of dissolved oxygen (DO) on the shortcut nitrification of aged landfill leachate and microbial community structure was investigated. The results showed that the maximum rate of nitrification was found in the group with low DO concentration (0.2-0.5 mg/L), after 17 h of reaction, the value of nitrate nitrogen/ammonia nitrogen was 1.05 and ammonia nitrogen load reached to 1.5 kg/(kg · d) (ammonia nitrogen consumed by per kg sus- pended solid every day), the effluent could meet the requirement of subsequent anaerobic ammonia oxidation treat ment. DGGE fingerprint atlas of the sludge revealed that the dominant bacteria in microbial community were uncul- tured Bacteroidetes bacterium, uncultured bacterium, uncultured Candidatus Amoebophilus sp. ect. ;DO had limited impact on bacterial community structure.