中文摘要：

目的掌握内蒙古自治区东部区通辽市主要产奶地区的牛乳中致病性金黄色葡萄球菌和无乳链球菌流行情况及其对常用抗生素药物的敏感性,为奶牛乳房炎的治疗提供参考依据。方法从通辽市周边奶牛场采集患乳腺炎的奶牛乳样19份,进行细菌培养和分离鉴定,对分离出的金黄色葡萄球菌和无乳链球菌菌株进行药物敏感性试验。结果19份奶样中共分离出24株金黄色葡萄球菌和22株无乳链球菌。药敏试验显示,分离的24株金黄色葡萄球菌菌株对氯霉素、氨苄青霉素的耐药率分别为95.8%和54.2%,而对链霉素等大多数抗生素均表现高度敏感或中度敏感;分离的22株链球菌对链霉素等7种抗生素的耐药率在50.0%～95.5%之间。结论内蒙古通辽市周边地区牛乳中分离的金黄色葡萄球菌对氯霉素、氨苄青霉素表现较高耐药性,分离的无乳链球菌对链霉素等抗生素普遍耐药,可能与抗生素滥用有关。

英文摘要：

Objective To determine the prevalence of Staphylococcus aureus and Streptococcus agalactiae and to analyze their susceptibility to antibiotics in order to provide evidence for control of bovine mastitis. Methods Milk samples were collected from 19 cows with mastitis around the City of Tongliao in eastern Mongolia. A bacterial culture was performed, and bacterial species were isolated and identified. Strains of Staphylococcus aureus and Streptococcus agalactiae were subjected to a drug sensitivity test. Results Twenty-four strains of Staphylococcus aureus and 22 strains of Streptococ- cus agalactiae were isolated from the 19 milk samples. Drug sensitivity testing indicated that 24 strains of Staphylococcus aureus were resistant to chloramphenicol at a rate of 95.8% and resistant to ampicillin at a rate of 54.2%. Staphylococcus aureus was highly or moderately sensitive to most antibiotics. Twenty-two strains of Streptococcus agalactiae were resistant to 7 antibiotics, including streptomycin, at a rate of 50.0%--95.5 %. Conclusion Staphylococcus aureus isola ted from bovine milk from areas of the City of Tongliao in Inner Mongolia had a high level of resistance to chloramphenicol and ampicillin, and Streptococcus agalactiae typically had a high level of resistance to antibiotics like streptomycin. This finding may be related to the overuse of antibiotics.