中文摘要：

目的：建立测定齐多夫定含量的新方法。方法：以甲基丙烯酸为功能单体,三甲氧基丙基三甲基丙烯酸酯为交联剂,合成抗HIV药物齐多夫定分子印迹聚合物,将其用于固相萃取,通过渗漏实验选择功能单体并考察了最大吸附量和最佳上样条件。结果：当以浓度为4.5 mmol·L^-1,pH=7的缓冲溶液上样时,最大吸附量达到59.7 mg·g^-1。并通过对上样、洗脱条件进行优化,成功地将齐多夫定从其结构类似物拉米夫定的混合物中分离出来。不同齐多夫定和拉米夫定加入量的回收率分别为101.6%～102.6%,103.4%～104.0%,RSD分别为1.6%～4.2%,1.4%～3.3%。结论：该方法快速、直观,可用于药物的分离与富集。

英文摘要：

Objective：To establish a new method for determination of zidovudine（AZT）.Methods：Using methacrylic acid as functional monomer,and trimethlolpropane trimethacrylate as crosslinking agent,molecularly imprinted polymers（MIP） specific for anti - HIV drug zidovudine were synthesised and packed into the SPE cartriges.Functional monomer,the maximum binding capacity and the best loading conditions were optimised by breakthrough experiment. Result：When the sample loaded at the concentration of 4.5 mmol·L^-1 NaH2PO4/NaOH buffer（pH 7.0）,the maximum adsorption capacity was up to 59.7 mg·g^-1 of polymers.The eluting condition was also optimized, zidovudine and its analog（lamivudine） were separated successfully by the MIP -SPE method.It was found that the proposed method can be used for the determination of AZT and 3TC in the mixture with good recoveries of 101.6%- 102.6%and 103.4%- 104.0%,RSDs are 1.6%-4.2%and 1.4%-3.3%.Conclution：The method is rapid and direct for separation and enrichment of drug.