中文摘要：

目的：建立测定奈韦拉平片中奈韦拉平含量的方法。方法：采用一阶导数UV光谱法,以275 nm（峰）与299 nm（谷）振幅值为定量依据,进行定量分析。该法与RP-HPLC法和零阶导数光谱法进行了对比。色谱柱为Shimadzu ODS（250 mm×4.6 mm,5μm）,流动相为甲醇-水（用磷酸调pH=3）,（60∶40）,流速为1.0 mL.min-1;紫外检测波长282 nm,柱温35℃,采用外标法计算含量。结果：结果表明奈韦拉平浓度在2.5～25.0μg.mL-1范围内与振幅值呈良好的线性关系（r=0.9997）,平均回收率为99.5%（RSD=1.5%）。统计检验发现HPLC法与一阶导数UV法无显著性差异,而与零阶导数法有显著性差异。结论：一阶导数UV光谱法可靠,简便快速,可有效消除赋形剂干扰,结果准确,可用于奈韦拉平片的质量控制。

英文摘要：

Objetive：To detemine the content of nevirapine in tablets.Methods：The first order derivative spectrometry for the determination of nevirapine was developed using amplitudes at 275 nm（peak） and 299 nm（valley）with comparable accuracy and precision to the RP-HPLC method and zero order derivative spectrometry,the quantification was carried out on a Shimadzu ODS column（250 mm × 4.6 mm,5 μm）,with mobile phase consisting of methanol and acetate buffer in the ratio of（60∶ 40）（pH = 3）.The mobile phase was pumped at a rate of 1 mL.min-1,at 35 ℃ with the detection at 282 nm.Results：The concentration of nevirapine within 2.5-25.0 μg.mL-1recovery was 99.5%（RSD = 1.5%）.Statistical test results showed that there is no significant difference between this method and RP-HPLC method,but the zero order derivative UV spectrometry method had significant difference with RP-HPLC method.Conclusions：This method was found to have suppressed the background absorption from the excipients with 12.5 μg.mL-1 presented a good linear relation（r = 0.9997） with the amplitudes and the average comparable accuracy and precision,and found to be simple,quick and accurate.It is suitable forquality control of this preparation.