目的:探讨京尼平固定神经生长因子(NGF)壳聚糖膜(GCN膜)缓释NGF的方法和作用。方法:实验组(GCN膜)采用纯天然生物交联剂京尼平将NGF固定在壳聚糖上,采用壳聚糖固定NGF以后的膜与未分化的PC12细胞共培养法观察细胞生长状态。另设阴性对照组(GC膜)不加NGF,阳性对照组(低血清F12K完全培养基中加入50ng/mlNGF)。结果:GCN膜组的PC12细胞在膜中释放出的NGF作用下突起生长明显,与阴性对照组有显著差异。结论:京尼平固定NGF壳聚糖膜可以有效缓释出具有生物活性的NGF,为临床上损伤神经的修复提供新的可能。
Objective: To study the effect of the controlled release of nerve growth factor (NGF) from genipin-crosslinked chitosan membranes immobilized with NGF(GCN) which were prepared in our lab on mutagenicity. Methods: The studies were conducted by cultured with the PC12 cells. Results: The neurite outgrowth of PC12 cells which were cultured on GCN membranes showed to be significantly higher than in the control groups. Conclusion: The GCN membranes can be materials for sustained release of bioactive NGF and provide a future possibility for therapeutic nerve repair.