中文摘要：

目的7日龄新生大鼠短暂脑缺血诱导侧脑室室管膜下区（SVZ）神经干细胞增殖，观察胰岛素样生长因子1（IGF-1）对神经干细胞增殖的影响。方法7日龄新生SD大鼠64只，随机分为缺血组（n=24）、假手术组（n=24）、缺血阻断剂组（n=8）和缺血生理盐水组（n=8）。利用免疫组织化学方法观察缺血组和假手术组在缺血后1、4、7dSVZ新生细胞数量和IGF-1表达的变化，并观察缺血阻断剂组和缺血生理盐水组在IGF-1受体阻断剂（JB1）阻断7d后SVZ新生细胞数量和IGF-1表达的变化。结果与同时间点假手术组比较，缺血后1、4、7d的SVZ新生细胞和IGF-1阳性细胞数量均显著增加，差异有统计学意义（P〈0．05）；使用JB1后，缺血阻断剂组IGF-1的表达被阻断，IGF-1阳性细胞缺如，而缺血生理盐水组IGF-1表达正常；使用JB1后，缺血阻断剂组SVZ新生细胞数量显著减少，与缺血生理盐水组相比，差异有统计学意义（P〈0．05）。结论新生大鼠缺血再灌注损伤上调了IGF-1的表达，IGF-1表达增加促使SVZ神经干细胞增殖；使用JB1后，IGF-1的表达被阻断，神经干细胞增殖也显著减少，提示IGF-1在脑缺血损伤修复中起关键作用。

英文摘要：

Objective To study the impact of insulin-like growth factor 1 （IGF-1） on the neural stem cell proliferation of the subventricular zone （SVZ） induced by transient forebrain ischemia in postnatal day 7 rats. Methods A total of 64 postnatal day 7 Sparuge-Dawley rats were randomly divided into ischemia group （ n = 24） , sham group（ n = 24） , ischemia plus antagonist group （ n = 8） and ischemia plus saline group （ n = 8）. The cell proliferation and IGF-1 in the SVZ of the ischemia group and the sham group were observed 1, 4 or 7 days after ischemia with immunohistochemical staining. The cell proliferation and IGF-1 in the SVZ of the ischemia plus antagonist group and the ischemia plus saline group were detected after 7 days of continuous treatment with JB1 or without JB1 treatment. Results The number of BrdU ＋ cells and IGF-1 ＋ cells of the ischemia group were significantly increased in the SVZ 1, 4, and 7 days after ischemia compared with that of the sham group （P 〈 0. 05）. After the administration of JBI, the IGF-1 expression was blocked. The IGF-1＋ cells were absent in ischemia plus antagonist group, while the IGF-1 expressed normally in ischemia plus saline group. The number of BrdU ＋cells in the ischemia plus antagonist was sharply decreased compared with the ischemia plus saline group （ P 〈 0.05 ） in the SVZ 7 days after ischemia. Conclusion Ischemia/perfusion up-regulates the expression of IGF-1 in neonatal rats, which may promote the proliferation of neural stem cells. Decrease of the neural stem cells proliferation dramatically after the administration of JB1 suggests that IGF-1 may play a key role on recovery of transient forebrain ischemia damage.