对黄姑鱼正常二倍体(N)、雌核发育二倍体(G)和单倍体(H)的胚胎发育进行观察,并对其早期生长情况进行比较。结果显示:(1)受精率N〉H〉G,孵化率N〉G〉H,畸形率H〉G〉N,72h成活率N〉G〉H;(2)正常二倍体胚胎经21h10min9呼化出膜,雌核发育二倍体和单倍体孵化出膜分别用时23h10min和23h30min;雌核发育二倍体发育滞后主要出现于原肠早期和孵出期,单倍体滞后出现于原肠晚期。从形态上看,单倍体胚胎呈现典型的单倍体综合症,雌核发育二倍体和正常二倍体胚胎发育形态正常且两者无明显差异。各组死亡高峰均出现在原肠晚期,单倍体组开口前全部死亡;(3)60日龄之前雌核发育二倍体生长速度明显慢于正常二倍体且个体间差异较大。
We observed the embryonic development of normal diploid (N), gynogenetic diploid (G) and haploid (H) of Nibea albiflora , and its early growth were compared. The results were as follows: (1) Fertilization rate N 〉 H 〉 G, Hatching rate N 〉 G 〉 H, Deformity rate H 〉 G 〉 N, 72 hours survival rate N 〉 G 〉 H. (2) It took about 21 hours 10 minutes for the normal diploid, for gynogenetic diploid and haploid, however, it took 23 hours 10 minutes and 23 hours 30 minutes to hatch, respectively. The embryonic development lag phenomenon were mainly appeared in early gastrula stage and hatching stage for gynogenetic diploid, in late gastrula stage for haploid. Morphologically, the haploid embryos showed typical haploid syndrome, while the gynogenetic diploid and normal diploid embryos had normal morphology and no significant difference between them. The death peak appeared in late gastrula stage for each group, all haploid larvae dead before mouth-open period. (3) Before 60 days age, the growth rate of gynogenetic diploid was obviously slower than normal diploid and individual quite different within the former group.