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中文摘要：
在流动的亚微米粒子的三维的追踪在一微隧道用同轴的 holographic 被执行显微镜学。A 修理了单身者 0.5 m 荧光灯粒子从粒子或重叠粒子配对使用导致的激光的灰尘被识别并且孤立荧光灯(LIF ) 方法。固定单个粒子的然后同轴的显微镜的全息图在光轴上在不同位置被获得，即 defocus 距离。单个粒子的全息图被用作模型模板与知道散焦距离。在在 microchannel 的流动的同轴的显微镜的全息图的粒子然后被识别并且定位了获得他们的二维的位置。那些粒子的 defocus 距离被匹配每个全息图模式到在单个粒子测试获得的模型模板之一决定。最后，每个粒子的三维的位置和速度被获得。
英文摘要：
Three-dimensional tracking of submicron particles in flows in a micro-channel was carried out using in-line holographic microscopy.A fixed single 0.5 μm fluorescent particle was identified and isolated from dust particles or overlapped particle pair using the laser induced fluorescent（LIF） method.Then in-line microscopic holograms of the fixed single particle were obtained at different positions on the optical axis,i.e.the defocus distances.The holograms of the single particle were used as the model templates with the known defocus distances.The particles in the in-line microscopic holograms of flow in the microchannel were then identified and located to obtain their two-dimensional positions.The defocus distances of those particles were determined by matching each hologram pattern to one of the model templates obtained in the single particle test.Finally the three-dimensional position and velocity of each particle were obtained.
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