中文摘要：

目的观察2型糖尿病KKAy小鼠的肝脏损害,研究黄芪和葛根素对其肝脏的保护作用。方法选取雄性KKAy小鼠,随机分为模型组、黄芪组、黄芪联合葛根素组;同龄雄性C57BL/J小鼠为正常组。检测空腹血糖。并分别于24、28周龄处死各组小鼠,检测甘油三酯（TG）、胆固醇（TCHO）、谷丙转移酶（ALT）、谷草转移酶（AST）等生化指标。制作肝脏病理切片,HE及油红O染色。结果模型组血糖、血脂、转氨酶较正常组明显升高,肝脏出现脂肪变性,肉眼可见模型组肝脏较正常组明显增大,触之油腻感,镜下可见肝细胞内大小不等的脂肪空泡。黄芪组及黄芪联合葛根素组,小鼠各项生化指标均有改善,尤其是AST、ALT显著降低,病理切片也显示肝脏结构较完整。结论 KKAy存在明显的肝损害及血脂异常,黄芪及黄芪、葛根素合用可有效抑制血脂异常,保护肝脏结构和功能。

英文摘要：

Objective To observe the liver injury in KKAy mice with type 2 diabetes,and study the protective effects of Huangqi（Radix Astragali seu Hedysari） and puerarin on liver.Methods Male KKAy mice were randomly divided into model group,Huangqi group and Huangqi combining puerarin group（combining group）.C57BL/J mice with the same age were selected as normal group.The change of fasting blood glucose（FBG） was detected.The levels of triglyceride（TG）,cholesterol（CH）,alanine aminotransferase（ALT） and aspartate aminotransferase（AST） were detected after mice were sacrificed respectively when they were 24 weeks old and 28 weeks old.The pathological sections of liver tissue were prepared and stained with HE staining and oil red O staining.Results In model group,the levels of FBG,blood fat,ALT and AST increased significantly than those in normal group,and there was steatosis in liver.The size of liver was larger by naked eye in model group than that in normal group and greasy by touch.There were empty lipocytes observed by microscope in different sizes in liver cells.In Huangqi group and combining group,all biochemical indexes were improved,especially the levels of AST and ALT decreased significantly,and pathological sections showed that liver structure was complete.Conclusion There are obvious liver injury and abnormal blood fat in KKAy mice,and Huangqi or Huangqi combining puerarin can effectively inhibit abnormal blood fat and protect the structure and function of liver.