中文摘要：

目的研究葛根素注射液对人近曲小管上皮细胞HK-2细胞株增殖的影响，以及对高糖诱导后细胞内78KD-糖调节蛋白（GRP78）表达的影响。方法设置葛根素质量浓度梯度，依次为5、10、20、40、80、160、320、640g／L，采用MTT方法，检测HK-2细胞的增殖情况。HK-2细胞传代贴壁后，无血清培养24h后，分为正常组、模型组和葛根素治疗组，继续培养48h后，提取各组细胞总RNA，采用RealTimePCR的方法，检测GRP78mRNA的表达情况。结果随着浓度的增大，葛根素注射液对HK-2细胞增殖的抑制作用增强。经葛根素注射液作用48h后，与正常组相比，模型组GRP78mRNA的表达量增多，差异有显著性（P〈0．01）；与模型组比较，治疗组GRP78mRNA的表达量增加，差异有显著性（P〈0．01）。结论葛根素注射液对HK-2细胞增殖具有抑制作用；高糖刺激HK-2细胞48h后，可导致其发生内质网应激反应，葛根素注射液可通过增加内质网伴侣蛋白GRP78的表达量，以促进蛋白质正确折叠，来恢复细胞的稳态。

英文摘要：

Objective To study the influences of Puerarin Injection on the proliferation of HK-2 cells and the expression of glucose regulated protein 78KD （GRF78） induced by high glucose. Methods The concentration gradient of puerarin was set as 5 g/L, 10 g/L, 20 g/L, 40 g/L, 80 g/L, 160 g/L, 320 g/L and 640 g/L. The proliferation of HK-2 cells was detected by using methyl thiazolyl tetrazolium test （MTI＇）. After passage and serum-free adhere-wall culturing for 24 h, HK-2 cells were divided into normal group, model group and treatment group （with Puerarin Injection） and were cultured continuously for 48 h. The total RNA were extracted from all groups and expression of GRP＇78 mRNA was detected by using real-time fluorescence quantitative polymerase chain reaction （RT-PCR）. Results The inhibitory effect of Puerarin Injection on the proliferation of HK-2 cells was reinforced as the increase of Puerarin Injection＇ s dose. After 48 h, the expression of GRP78 mRNA increased in model group compared with normal group （P 〈0.01 ） , and the expression increased in treatment group compared with model group（P 〈 0.01 ）. Conclusion Puerarin Injection has inhibitory effect on the proliferation of HK-2 cells. After high glucose stimulation for 48 h, ERS stress reaction will be induced, and Puerarin Injection can improve correct protein folding and recover intracellular homeostasis through increasing the expression of GRP78 mRNA.