中文摘要：

目的从肾功能和肾组织形态改变方面观察黄芪注射液联用葛根素注射液对2型糖尿病KKA，小鼠肾脏的保护作用及二者对肾组织纤黏连蛋白（fibronectin，FN）的表达影响。方法将雄性KKA^y小鼠分为模型组、黄芪注射液联合葛根素注射液治疗组，同龄雄性C57BL／6J小鼠为正常对照组。定期监测血糖、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、血清尿素及尿酸，HE及Masson染色观察肾组织病理变化，免疫组化观察肾组织FN表达情况。结果从20周龄开始，模型组小鼠空腹血糖、血脂、血清尿素及尿酸较正常组明显升高，治疗组小鼠上述指标较模型组明显改善。HE及Masson染色见模型组肾小球硬化，肾小管上皮细胞胞浆空泡明显，肾间质胶原组织增多，治疗组小鼠以上病变明显减轻；免疫组化显示模型组FN表达较正常组明显升高，治疗组各周龄小鼠肾脏组织FN的表达与模型组比较均减少。结论黄芪注射液联用葛根素注射液能改善2型糖尿病KKA^y小鼠的肾功能，减轻肾脏的病理改变，减少系膜区FN的表达，具有保护肾脏作用。

英文摘要：

Objective To observe the protective effects of Huangqi Injection and Puerarin Injection on kidney and their influences on expression of fibronectin （FN） in KKA^y mice with type 2 diabetes based on the changes of kidney function and renal morphology. Methods Male KKAy mice were divided into model group and treatment group （treated with Huangqi Injection combining Puerarin Injection）, and male same- aged C57BL/6J mice were taken as normal group. The levels of plasma glucose, LDL-C, HDL-C and serum urea and uric acid （UA） were regularly detected. The pathological changes of renal tissue were observed after HE and Masson staining, and FN expression was observed by using immunohistochemistry technique. Results Started from 20 weeks old, the levels of plasma glucose, blood fat and serum urea and （UA） increased significantly in model group than those in normal group, and improved in treatment group compared with model group. HE and Masson staining showed glomerular sclerosis, vacuolar degeneration in renal tubular epithelial cells and more interstitial collagen in model group, and these pathological changes were significantly relieved in treatment group. The results of immunohistochemistry technique showed that FN expression increased significantly in model group than that in normal group, and decreased in treatmentgroup than that in model group. Conclusion Huangqi Injection combining Puerarin Injection can improve kidney function, alleviate renal pathological changes, reduce FN expression and protect kidney in KKA^y mice with with type 2 diabetes.