中文摘要：

目的探讨靶向沉默组蛋白去乙酰化酶2（histonedeacethylase2，HDAC2）对人肝癌细胞HepG2增殖的影响。方法根据HDAC2的碱基序列设计并合成小干扰RNA（smallinterferingRNA，siRNA），通过脂质体Lipofectamine。”2000转染到HepG2细胞内，荧光倒置显微镜观察细胞的转染效率，用四甲基偶氮唑盐（MTr）法检测HepG2细胞的增殖变化；流式细胞术检测HepG2细胞周期变化；RT—PCR检测HDAC2、CyclinD1、CDK4mRNA的表达；Westernblot检测HDAC2、CyclinD1、CDK4蛋白的表达。结果将HDAC2-siR—NA转染进HepG2细胞内，HDAC2基因及蛋白水平明显降低；同时CyclinDl、CDK4mRNA及蛋白水平亦明显降低；靶向封闭HDAC2基因的表达可明显抑制HepG2细胞的增殖。结论靶向封闭HDAC2基因的表达可明显抑制HepG2细胞的增殖，HDAC2可能是潜在抗癌药物的治疗靶点。

英文摘要：

Aim To investigate the effect of cell prolif- eration in human HepG2 cells by RNA interference in- hibiting expression of HDAC2 gene. Methods siRNA targeting HDAC2 was designed and synthesized accord- ing to its mRNA, and their corresponding expression vectors were constructed and transfected into human HepG2 cells with lipofectaminelTM 2000. Then the cor- puscular transfection efficiency was observed by invert- ed microscope. Cell proliferation was measured by MTT assay and cell cycle indexs were detected by flow cytometry. Changes of CyclinD1, CDK4 and HDAC2 mRNA were analyzed by RT-PCR. The protein level of CyclinD1 ,CDK4 and HDAC2 was measured by West- ern blot. Results HDAC2 siRNA effectively inhibited cell proliferation, and HDAC2 siRNA significantly de- creased the expression of CyclinD1, CDK4 and HDAC2, suggesting that HDAC2 may be a potential target gene in human hepatocellular carcinoma. Con- clusions Silencing HDAC2 expression by siRNA significantly inhibits human HepG2 cell proliferation. HDAC2 may be a potential therapeutic target gene for human hepatocellular carcinoma.