中文摘要：

目的利用小干扰RNA（siRNA）抑制TRPM7基因的表达，研究TRPM7对Aβ25-35诱导SH-SY5Y细胞分泌炎症因子的影响。方法通过Aβ25-35 诱导SH-SYSY细胞构建阿尔采末病体外模型，MTT 法测定Aβ25-35 诱导SH-SY5Y细胞最佳作用浓度与时间点，将人工合成抑制TRPM7基因的siRNA片段，通过脂质体Lipofectamine^TM 2000转染到SH-SYSY细胞内；RT-PCR检测TRPM7、NF-κB、TNF-α、IL-6mRNA表达，乳酸脱氢酶（LDH）试剂盒的测定细胞上清乳酸脱氢酶（LDH）的含量；ELISA测定细胞上清中TNF-α、IL-6的含量。结果转染siRNA后SH-SYSY细胞的TRPM7、NF-κB、TNF-α、IL-6mRNA表达较模型组明显下降，细胞上清中TNF-α、IL-6、LDH的含量表达较模型组明显下降。结论应用siR-NA干扰靶向抑制TRPM7基因，可以下调TNF-α、IL-6等炎症因子的释放，这一过程可能是通过抑制NF-κB信号转导通路的激活，从而减少炎症因子的释放，进而减轻AB对sH-SYSY细胞的毒性作用。

英文摘要：

Aim To investigate the effects of down-reg- ulated TRPM7 gene expression by small interference RNA（ siRNA ） on the expression of inflammatory fac- tors in SH-SY5Y cells induced by β-amyloid pep- tide（25-35）. Methods Alzheimer＇s disease in vitro model was constructed by inducing SH-SY5Y cells with Aβ25-35. Then MTT method was used for determining the best effective concentration and time point of Aβ25-35 induced SH-SY5Y cells. And the sequence of TRPM7 siRNA was chemically synthesized. The siRNA was transfected into SH-SYSY cells by LipofectamineTM 2000 to down-regulate TRPM7 gene. Expressions of TRPM7 ,NF-κB,IL-6, TNF-α mRNA were analyzed by RT-PCR. Lactic acid dehydrogenase （LDH） kit was used to determine the lactate dehydrogenase （LDH） content of cell supernatant. The concentration of TNF-ct and IL-6 in cell supernatant was assayed by ELISA .Results After the SH-SY5Y cell was transfected with siRNA, TRPM7, NF-κB, IL-6, TNF-α mRNA expres- sion decreased significantly compared with the model group. And expressions of cell supernatant of TNF-α and IL-6 content were also decreased significantly. Conclusion Targeted inhibition of TRPM7 gene by siRNA interference can down-regulate the expression of inflammatory factors, such as TNF-α, IL-6. This process may be accomplished by inhibiting the activa- tion of NF-κB signal pathway to down-regulate the ex- pression of inflammatory factors and then reduce the cytotoxicity on SH-SYSY cells induced by β-amyloid peptide.