长期的保藏和人的骨头的容易的交通导出髓的间充质的干细胞(hBM-MSCs ) 将在医疗和生物工程学便于他们的申请。hBM-MSCs 的 freeze-drying 上的一个预研项目被执行。hBM-MSCs 与 trehalose 被装载。freeze-drying 暂停的玻璃转变温度被测量为冷却和主要弄干实验提供信息。在 freeze-drying 以后,各种各样的水和过程被测试。hBM-MSCs 的最高的恢复率是(69.33 phosphorylase 是涉及 nucleosides 的生合成的重要的酶。在这研究,嘌呤 nucleoside phosphorylase 和嘧啶 nucleoside 黄磷?
Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was (69.33±13.08)%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.