中文摘要：

目的：研究山茱萸环烯醚萜苷（CIG）对蛋白磷酸酶抑制剂冈田酸（OA）拟阿尔茨海默病（AD）细胞模型中神经细胞凋亡的影响及其作用机制。方法：CIG与人神经母细胞瘤细胞系SK—N—SH细胞预孵育24h，再用OA 10nmol与神经细胞共孵育6h，用TUNEL法观察凋亡细胞的变化，用蛋白免疫印记法观察凋亡因子B细胞淋巴瘤（Bcl-2）、Bcl-2相关X蛋白（Bax）和细胞凋亡蛋白酶（Caspase-3）的表达。结果：正常对照细胞铺展良好，未出现凋亡细胞；OA模型组凋亡细胞显著增多，Bax和Cas～pase3的表达水平显著增高，Bcl-2的表达水平显著下降；CIG（100和200μg·mL^-1）给药组凋亡细胞较OA模型组显著减少，Bax和Caspase-3表达水平显著降低，Bcl-2表达显著增高。结论：CIG能通过影响凋亡调节因子而抑制神经细胞凋亡，提示该药可能具有治疗AD的艮好应用前景。

英文摘要：

OBJECTIVE： To investigate the effects and the mechanisms of cornel iridoid glycoside （CIG） on antiapoptosis in cellular model of alzheimer disease（AD） induced by the protein phosphatase inhibitor okadaic acid （OA）. METHODS： The human neuroblastoma cell line SK N- SH cells were preincubated with CIG for 24 h, and then incubated with OA 10 nmol for 6 h. The changes of apoptotic cells were observed by TUNEL method. Western blotting was used to determine the expression of apoptosis-regulating factors Bcl - 2, Bcl 2 related X protein（Bax） and Caspase - 3. RESULTS： The normal SK - N - SH cells spread well and no apoptotic cells appeared. In OA-treated model group, the number of apoptotic cells increased signif- icantly, the expressions of Bax and Caspase- 3 increased significantly, but Bcl- 2 expression decreased significantly. In CIG （100 and 200μg · mL^-1） -treated group, the number of apoptotic cells decreased significantly as compared with OA- treated model group, and the expressions of Bax and Caspase- 3 decreased significantly whereas Bcl 2 expression increased significantly. CONCLUSION： CIG can inhibit the apoptosis of nerve cells through affecting apoptosis-regulating factors, suggesting its potential as a therapy for AD.