中文摘要：

采用平板分离和培养瓶厌氧技术，结合 PCR 扩增技术，以沙曲矿煤为发酵基底，中泰矿业和沙曲矿井水及白腐菌为菌种来源，进行产甲烷菌培养实验，并添加不同浓度梯度的 EDTA 二钠，进行产甲烷菌培养实验，测定实验结束后的产甲烷量。实验证明：中泰矿业矿井水中有产甲烷菌的存在，而沙曲矿井水在含反硝化菌和 pH 偏高的情况下，抑制了产甲烷菌的生长。同等条件下沙曲矿批次实验中没有甲烷气体的产生，而中泰矿业的有气体产出，适宜浓度的 EDTA 二钠浓度会显著提高产气量，EDTA 二钠用量为1.0 g/ L 时，中泰矿井水 CH4产率达到最大，为10.0 mL/ g 煤，反应后溶液的 pH 值明显增高。

英文摘要：

Adopting the plate separation technology and incubation bottles anaerobic technology,combined with the PCR amplification technology,using coal samples from Shaqu coal mine as the fermentation substrates and the mine water respectively from Zhongtai Mining and Shaqu mine and white rot fungi as the strain sources,the methane-produ-cing bacteria cultivation experiment was conducted and the methane production was determined by adding the bi-sodi-um EDTA solution with various concentration gradients. The experimental had confirmed that there exist methane-pro-ducing bacteria in the mine water of Zhongtai Mining,and under the conditions of the content of denitro-bacteria and slantly high pH in the mine water of Shaqu coal mine the growth of methane-producing bacteria was inhibited. Under equal conditions,there is no methane produced in the batch experiments coal samples from Shaqu mine,while there is a gas output in the samples of Zhongtai Mining,and appropriate concentration of bi-sodium EDTA significantly in-creased the gas production. When the bi-sodium EDTA was at 1. 0 g/ L,the CH4 production rate reached the maxi-mum at 10. 0 mL/ g,and the pH value increases notably after the reaction.