中文摘要：

以沙曲矿焦煤为发酵底物，以同矿区矿井水中的菌种及实验室保存的白腐菌为投加菌种，研究在35℃，初始pH为6．5，无光照和厌氧条件下不同发酵工艺对煤发酵制氢的影响．结果表明，在所设条件下，Hz的体积分数最高可达73．79／6，产率可达23．56mL／g；未添加白腐茵液样品的H：产率是添加了白腐茵液样品的1．5～6倍；其他条件相同，提前4d富集矿井水中的发酵产氢茵可以将Hz产量从0．44mL提高到466．34mL；在富集矿井水中发酵产氢茵时添加EDTA二钠要比富集完之后再补加的H。产率高2～10倍．可见煤通过微生物作用完全可以制取更加洁净的能源——氢．

英文摘要：

As we know, coal could be transformed into biogenic methane by microbiologi- cal action, but biogenic hydrogen production from coal has not yet been reported. This study will constitute an important complement to the coal bed methane bioengineering. Taking coking coal in Shaqu mine as fermentative substrate; taking mine water strains in the same mining area and the white-rot fungus preserved in laboratory as bacterial source, the impact of bio-hydrogen production by different fermentation technology was studied at conditions which temperature is 35 ℃, initial pH is 6.5 without illumination and anaerobic environment. The results show that. under the conditions of the experiment, the hydrogen volume fraction could reach to 73.7%, the hydrogen production rate could achieve 23.56 mL/g coal; compared to the samples that didn＇t mix white-rot fungus, the hydrogen production rate of the samples which added the white rot fungus were decreased 1.5-6 times; other things being equal, hydrogen production could be in- creased from 0.44 mL to 466.34 mL if hydrogen producing bacteria in mine water were enriched for 4 d; compared mixed edentate disodium at the beginning of enrich hydrogen producing bacteria with adding it after enrich strains, the former hydrogen production rate were 2-10 times than the latter. Obvi- ously, more clean energy-hydrogen could be obtained from coal by microbiological action.