中文摘要：

本试验根据梅花鹿朊蛋白基因序列设计引物,利用PCR的方法从梅花鹿基因组DNA中扩增朊病毒蛋白酶抵抗区域PrPres,将该片段分别与表达载体pET-Trx和pET-His连接,构建重组表达载体pET-Trx-PrPres和pET-His-PrPres。分别将两个重组表达载体转入E.coli BL21（DE3）plys宿主菌中,37℃诱导4h,经SDS-PAGE分析,Trx-PrPres和His-PrPres表达量分别为38.2%和30.1%。

英文摘要：

The gene of prion protein protease resistance fragment PrPres of the Sika deer was amplified by PCR using a pair of specific primers.The product of PCR was cloned into the expression vectors of pET-Trx and pET-His.The restructuring plasmids were transformed into the host bacterium of E.coli BL21（DE3） plys,at 37 ℃ for 4 h.SDS-PAGE result illustrated that fusion proteins of Trx-PrPres and His-PrPres were highly expressed.The expression level of fusion proteins were 38.2% and 30.1%,respectively.