中文摘要：

目的研究在三维细胞培养条件下,电离辐射诱导人成纤维细胞发生适应性反应。方法采用人成纤维细胞进行三维条件培养,以60Co进行照射（诱导剂量D1为0.1Gy,攻击剂量D2为2Gy）,以Western blot检测生长相关基因p53和p21的表达,免疫荧光染色及共聚焦法观察形态学变化。结果三维培养条件下,细胞生长与二维（单层）培养形态学表现明显不同,细胞形态伸展,呈现伪足和多微丝状,细胞正常∶异常∶中间态比例为87.05%∶6.1%∶6.85%。经过2Gy（D2）照射后细胞明显收缩,伪足和微丝消失,与对照组比较,细胞变为正常∶异常∶中间态比例30.4%∶67.45%∶2.15%;预先给以0.1Gy即D1＋D2照射后则出现异常细胞数量减少,正常细胞有所增加,同时不典型的中间态细胞比例增加,细胞比例为正常∶异常∶中间态35.5%∶34.7%∶30.4%。结论人成纤维细胞在三维培养条件下,预先低剂量60Co照射可以降低随后高剂量照射引起的形态学改变,诱导产生适应性反应。

英文摘要：

Objective To observe the adaptive response induced by ionizing radiation in human fibroblasts under 3-dimentional culture condition. Methods Human fibroblasts were cultured under 3-D condition. Western blot was used to detect the expressions of p53 and p21. Immunofluroescence and confocal microscopy were used to detect the morphological changes. Results The structures of the cells under 2-D and 3-D conditions were fundamentally different,the cells under 3-D condition stretched out and formed microspikes, the proportions of normal, abnormal and in between were 87.05 %, 6.1% and 6.85 %, respectively. After the treatment of 2 Gy（ D2 ） most of the fibroblasts shrinked and the microspikes disappeared, while the pre-tretment of 0.1 Gy（ D 1 ） pulsing the challenging dose of 2 Gy（ i. e. , D 1 ＋ D2 ） caused the decrease of abnormal cells and the increase of the normal cells and in between（35.5 % ：34.7 % ：30.4 % ）. Conclusions Under the 3-D conditions pretreatment with low dose radiation could attenuate the morphological changes induced by high dose. i. e. induced the adaptive response.