中文摘要：

目的：观察经侧脑室注射S100A4蛋白对脑梗死大鼠神经功能以及脑内S100B蛋白表达的影响。方法：36只健康成年SD大鼠线栓法成功复制大鼠大脑中动脉闭塞模型（MCA0），随机分为A、B组各18只，术后第1天A组侧脑室行人工脑脊液注射；B组行S100A4蛋白注射。在MACO术后第1、7及14d时各组各取6只行神经行为学评分，免疫组织化学法检测脑内S100B蛋白的表达情况。结果：B组大鼠在术后第7，14天时神经行为学评分与术后第1天比较有明显下降（1．96±0．49、1．75±0．40与2．94±0．72，P〈0．05），并低于相同时间点A组大鼠。S100B阳性细胞数在术后第7天2组脑内达到峰值，以后开始减少，2组间比较，B组少于A组（P〈0．05）。结论：侧脑室注射S100A4蛋白可一定程度改善脑梗死大鼠神经行为学功能缺损，减少S100B蛋白在脑内的表达。

英文摘要：

Objective ： To observe the effects of injecting S100A4 into the lateral ventricles on neural function and expression of S100B in rats with cerebral infarction. Methods： Thirty-six Sprague Dawley （SD） rats were randomly divided into two groups： artificial cerebrospinal fluid （aCSF） group （18 rats） and S100A4 protein group （18 rats）. The animal model was made by occluding middle cerebra artery. Behavior scores of each group were recorded on the 1st, 7th, and 14th day after operation. Immunohistochemical technique was used to detect the expression of S100B protein in the brain tissue. Results： Neural function scores on the 7th and 14th day in S100A4 protein group were significantly reduced as compared with those on the 1st day, as well as those in aCSF group （P〈0.05）. The number of S100B positive cells in the brain tissue was significantly increased on the 1st and 7th day, and decreased after the 7th day both in S100A4 protein group and aCSF group. The number of S100B positive cells in S100A4 protein group was less than that in aCSF group （P〈0.05）. Conclusion： S100A4 protein can improve neural functions and reduce S100B expression in rats with cerebral infarction, indicating that S100A4 protein could be a neuroprotectant in vivo.