中文摘要：

以从水稻T-DNA转化群体中筛选出的可转绿和不可转绿两种白化株系为研究对象,分析了叶片中叶绿素及其合成途径中δ-氨基乙酰丙酸（ALA）、胆色素原（PBG）、尿卟啉原Ⅲ（UroⅢ）、原叶绿素（酸）（Pchl）、原卟啉IX（Proto-IX）和镁原卟啉Ⅸ（Mg-ProtoⅨ）等6种中间产物的含量。结果发现,幼苗期可转绿和不可转绿株系叶片中均无叶绿素;在三叶期,可转绿白化株系逐渐转绿、叶绿素含量也很快增加,而不可转绿株系仍为白色、无叶绿素含量。经测定,中间产物含量推测不可转绿白化株系叶绿素合成受阻位点可能在PBG与UroⅢ之间,而可转绿白化株系叶绿素合成受阻位点可能在Pchl和Chla之间。

英文摘要：

Two kinds of albino plantlets selected from T-DNA insert lines were used in this study,and the contents of chlorophyll and intermediates of chlorophyll synthesis such as ALA,PBG,UroⅢ,Pchl,Proto-IX and Mg-Proto Ⅸ were investigated.The results showed that there was no chlorophyll in the leaves of the albino plantlets before three-leaf stage.From three-leaf stage,the leaves of the green-revertible albino line turned green and showed chlorophyll accumulation,while the leaves of the permanent albino line were white and no chlorophyll accumulation.According to the content of the 6 intermidiates,we suggested that the obstacle occurred between PBG and Uro Ⅲ in the permanent albino line,while the obstacle occurred between Pchl and Chla in the green-revertible albino.