目的:研究不同温度对苍术生长发育及挥发油组分的影响。方法:设置低温组(T1)、适宜温度组(T2)、高温组(B)3组,各重复8个,生长6个月,收苗。采用t检验比较生物量;GC—MS分析、鉴定苍术根茎挥发油中所含的有机化合物;BIOLOG微平板每孔颜色平均变化率检测苍术根际微生物群落功能多样性。以此观察苍术挥发油组分及根际环境的综合变化。结果:①不同温度下生长的苍术的株高和总生物量有显著差异(P〈0.05)。②不同温度下苍术根茎挥发油中归一化百分含量大于0.01%的组分相差较大。三者共有组分有7个。其中T1处理鉴定出17个组分,T2处理鉴定出21个组分,B处理鉴定出21个组分;挥发油总含量(mL·g^-1):T1(23.0597)〈T2(24.7491)〈T3(26.1917)。③T1和L使得SOD升高,T1使得CAT降低,T3和T1均使POX升高。④不同温度下苍术土壤微生物群落BI—OLOG代谢剖面(AWCD)发生一定程度的变化,其变化程度与培育的时间及不同温度环境有关。T1、T3条件的AWCD较T2低。结论:不同温度对苍术生长发育、挥发油成分、叶片酶活性和土壤微生物群落均有影响。
Three treatment groups were set up to study the effects of different temperatures on the growth and components of essential oil of Atractylodes lancea (Thunb.) DC., namely low temperature (T1), suitable temperature (T2) and high temperature (T2), each treatment repeated for eight times. The plants were harvested aftet six months. The total biomass was compared by t test, the method of GC-MS was conducted for the analyses and identification of the compounds in the essential oil of rhizoma. The functional diversity of rhizosphere about microbial community was tested by BIOLOG. The comprehensive change was observated. The results of statistical analysis were as follows: 1) Differences in the plant height and total biomass were all statistically significant(P〈0.05). 2) GC-MS analysis of the essential oil in the underground part of the plants showed that, under different temperatures, the percentages 〉 0.01% of the essential oil in the rootstalk of the plants were different. Seven components were simultaneously found in all groups. T1 identified 17 components, T2 22 components, and T3 21 components. The total content of essential oil (mgog-1) ranked in the order of TI (23.0597)〈T2(24.7491)〈T3(26.1917). 3) T1 and T3 increased the superoxide dismutase(SOD) activity, TI decreased catalase (CAT) activity, and both T1 and T3 increased peroxide(POX) activity. 4) The average well color development (AWCD) of soil microbe community changed in varying degrees under different temperatures, depending upon the cultivation time and temperature. The Shannon's diversity index, Shannon's evenness index, McIntosh's diversity index and McIntosh's evenness index of the soil environment changed in the same tendency. The AWCD value was relatively low under the condition of TI and T3 than under that of T2. All the above results indicate that the temperature stress has certain effects on the growth of A. lancea, the components of its essential oil, the activity of