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不同温度对茅苍术生长发育及挥发油组分的影响
  • ISSN号:1674-3849
  • 期刊名称:《世界科学技术:中医药现代化》
  • 时间:0
  • 分类:R284.2[医药卫生—中药学;医药卫生—中医学]
  • 作者机构:[1]中国中医科学院中药研究所,北京100700, [2]中国科学院生态环境中心,北京100085, [3]北京市农业局小汤山特菜基地,北京102211
  • 相关基金:国家自然科学基金项目(30672616):环境胁迫下AM影响苍术质量的机理研究,负责人:郭兰萍;国家自然科学基金项目(30672617):基于3S技术的中药道地性研究及空问信息分析数据库的构建,负责人:郭兰萍;国家科技支撑计划(2006BAI09803):有效恢复中药材生产立地条件与土壤微生态环境修复技术研究,负责人:郭兰萍;国家中医药管理局行业专项(200707014):苍术等道地药材鉴别特征提取,负责人:郭兰萍;国家重点基础研究发展计划(2006CB504700):中药药性理论继承与创新研究,负责人:黄璐琦.
中文摘要:

目的:研究不同温度对苍术生长发育及挥发油组分的影响。方法:设置低温组(T1)、适宜温度组(T2)、高温组(B)3组,各重复8个,生长6个月,收苗。采用t检验比较生物量;GC—MS分析、鉴定苍术根茎挥发油中所含的有机化合物;BIOLOG微平板每孔颜色平均变化率检测苍术根际微生物群落功能多样性。以此观察苍术挥发油组分及根际环境的综合变化。结果:①不同温度下生长的苍术的株高和总生物量有显著差异(P〈0.05)。②不同温度下苍术根茎挥发油中归一化百分含量大于0.01%的组分相差较大。三者共有组分有7个。其中T1处理鉴定出17个组分,T2处理鉴定出21个组分,B处理鉴定出21个组分;挥发油总含量(mL·g^-1):T1(23.0597)〈T2(24.7491)〈T3(26.1917)。③T1和L使得SOD升高,T1使得CAT降低,T3和T1均使POX升高。④不同温度下苍术土壤微生物群落BI—OLOG代谢剖面(AWCD)发生一定程度的变化,其变化程度与培育的时间及不同温度环境有关。T1、T3条件的AWCD较T2低。结论:不同温度对苍术生长发育、挥发油成分、叶片酶活性和土壤微生物群落均有影响。

英文摘要:

Three treatment groups were set up to study the effects of different temperatures on the growth and components of essential oil of Atractylodes lancea (Thunb.) DC., namely low temperature (T1), suitable temperature (T2) and high temperature (T2), each treatment repeated for eight times. The plants were harvested aftet six months. The total biomass was compared by t test, the method of GC-MS was conducted for the analyses and identification of the compounds in the essential oil of rhizoma. The functional diversity of rhizosphere about microbial community was tested by BIOLOG. The comprehensive change was observated. The results of statistical analysis were as follows: 1) Differences in the plant height and total biomass were all statistically significant(P〈0.05). 2) GC-MS analysis of the essential oil in the underground part of the plants showed that, under different temperatures, the percentages 〉 0.01% of the essential oil in the rootstalk of the plants were different. Seven components were simultaneously found in all groups. T1 identified 17 components, T2 22 components, and T3 21 components. The total content of essential oil (mgog-1) ranked in the order of TI (23.0597)〈T2(24.7491)〈T3(26.1917). 3) T1 and T3 increased the superoxide dismutase(SOD) activity, TI decreased catalase (CAT) activity, and both T1 and T3 increased peroxide(POX) activity. 4) The average well color development (AWCD) of soil microbe community changed in varying degrees under different temperatures, depending upon the cultivation time and temperature. The Shannon's diversity index, Shannon's evenness index, McIntosh's diversity index and McIntosh's evenness index of the soil environment changed in the same tendency. The AWCD value was relatively low under the condition of TI and T3 than under that of T2. All the above results indicate that the temperature stress has certain effects on the growth of A. lancea, the components of its essential oil, the activity of

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期刊信息
  • 《世界科学技术:中医药现代化》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中科院科技政策与管理学研究所
  • 主编:陈凯先
  • 地址:北京市海淀区中关村东路55号中科院基础园区思源楼1203室
  • 邮编:100190
  • 邮箱:wst@casipm.ac.cn
  • 电话:010-62616352 62652762
  • 国际标准刊号:ISSN:1674-3849
  • 国内统一刊号:ISSN:11-5699/R
  • 邮发代号:2-534
  • 获奖情况:
  • 2009年获得中国科学院科技期刊基金三等奖,2005-2011年连续获得科技部“中国科技核心期刊”奖,2007-2011年与ELSEVIER合作,成为ScienceDirect英...,2009年获得国家科技部等14部委主办的“2009传统医...
  • 国内外数据库收录:
  • 中国中国科技核心期刊,中国北大核心期刊(2011版)
  • 被引量:12547