中文摘要：

目的： 建立藏药桃儿七的HPLC指纹图谱，比较不同产地桃儿七成分含量差异，进行产地识别，综合评价桃儿七质量。方法： 采用HPLC，Agilent Zorbax SB C18柱（4.6 mm×250 mm，5μm），乙腈-0.04%甲酸水为流动相梯度洗脱，流速1.0 mL·min-1，柱温30℃，对16批桃儿七样品进行指纹图谱测定，并使用主成分分析和聚类分析对指纹图谱进行产地识别和质量评价。结果： 建立了桃儿七药材指纹图谱；对16批样品相似度比较，标定了29个共有峰；各产地样品成分组成基本相同，但是含量存在一定差异；对各样品进行化学模式识别，根据主成分得分，产自西藏的药材质量最好。结论： 该方法能够用于桃儿七药材综合评价及质量控制。

英文摘要：

Objective： To establish the fingerprint of Sinopodophyllum hexandrum from different origin by HPLC, and compare the durg through the Chemical Pattern Recognition to provide the basis on comprehensive evaluation of the quality of S. hexandrum. Method： Based on HPLC method, Agilent Zorbax SB C18 column （4.6 mm×250 mm, 5μm） was used with acetonitrile-0.04% formic acid as the mobile phase by gradient elution. Flow rate was 1.0 mL·min-1; the column temperature was set at 30℃. 16 samples of S. hexandrum fingerprint were compared. Chemical pattern Recognition Method of S. hexandrum cluster was used to analyze on the origin and evaluate quality. Result： S. hexandrum fingerprint by HPLC was established with comparison of similarity for 16 batches of samples, calibration of the 29 common chromatographic peaks.The composition of each sample was the same, but the content was quite different. Based on the principal component analysis, the medicinal materia from Tibet was the best. Conclusion： The method can be used for S. hexandrum comprehensive evaluation and quality control.