为研究江苏沿江地区糯玉米种质的遗传多样性以及江苏省糯玉米的遗传改良和杂种优势利用提供参考,利用93对SSR标记研究55份糯玉米自交系和30份糯玉米单交种的遗传多样性,并利用UPGMA方法对所有自交系材料进行系统聚类。结果表明在自交系中筛选出88对多态性较好的引物,共扩增出350个差异片断,每对引物检测出2~9个差异片段,平均为3.98个;SSR标记的多态性信息量值在0.137与0.832之间,平均为0.520。在单交种中筛选出87对多态性好的引物,扩增出311个差异片断,每对引物可检测出2~9个差异片断,平均3.57个,SSR标记的多态性信息量值在0.064与0.839之间,平均为0.475。利用UPGMA聚类分析方法将所有供试自交系分为4类,划分结果基本符合品系的来源情况,江苏沿江地区糯玉米种质资源主要由通系5群、衡白522群以及突变体材料组成,多样性较为丰富,可为糯玉米遗传改良提供一定的遗传基础。
Germplasm resources are vital for genetic improvement.A narrow germplasm base will restrict plant breeding,and reduces the potential for improving heterosis and the possibility of enhancing resistance to biological or non-biological pressure.The Jiangsu coastal district is one of the regions where waxy corn breeding has been earliestly developed.However,the genetic diversity of breeding resources was still unknown.In this study,a total of 85 waxy corn resources(55 waxy corn inbreds and 30 hybrids) in this area were collected as a panel,and the genetic diversity was studied by 93 pairs of SSR primers distributed on 10 chromosomes of maize.88 pairs of primers were polymophic in inbred lines,and each could stably amplify 2-9 differential fragments with an average of 3.98.The value of polymorphism information content(PIC) for each SSR locus in inbred lines varied from 0.137 to 0.832 with an average of 0.520.In hybrids,87 primers produced 311 polymorphic fragments.The number of polymorphic fragments amplified by each primer varied from 2 to 9 with an average of 3.57.The value of polymorphism information content for each SSR locus in hybrids varied from 0.064 to 0.839 and the average PIC for SSR loci was 0.475.The 55 inbreed lines were classified into 4 groups using UPGMA method,which agreed with the pedigree.Tongxi 5 subgroup,Hengbai 522 subgroup and some mutants dominated the waxy corn resources in this area.