中文摘要：

通过正交试验设计优化钙离子交联法制备羧甲基壳聚糖纳米粒工艺条件,以透射电镜观察,纳米粒外观形态圆整;以激光粒度分析仪测定,纳米粒平均粒径为（131.2±5.27）nm;以高效液相色谱法测定,纳米粒包封率为（51.2±0.41）%,载药量为（16.7±0.29）%。对模型药物甘草酸的体外释放性能考察结果表明,所制备的纳米粒具有较好的控制药物释放的作用。

英文摘要：

The carboxymethyl chitosan nanoparticles were prepared through ionic gelification with calcium ions. The orthogonal test design was used to optimize the preparation process. The shape of the nanoparticles were studied by TEM. Nanoparticle size was evaluated by laser particle size determination. The high performance liquid chromatography was used to detect the drug-loading rate and entrapment efficiency of particles, and the in vitro release was investigated. The results show novel nanoparticles were spherical, average in particle size （131.2±5.27）nm, entrapment efficiency （51.2±0.41）%, drug-loading capacity （16.7±0.29）%, sustained release of carboxymethyl chitosan nanoparticles is significant.