中文摘要：

目的：研究腺病毒介导入VEGF-siRNA对裸鼠移植骨肉瘤的治疗作用。方法：利用腺病毒重组技术将VEGF—siRNA基因克隆入增殖缺陷型腺病毒基因组中，构建重组腺病毒Ad—VEGF-siRNA，重组腺病毒体外感染骨肉瘤MG63细胞，RT-PCR检测MG63细胞VEGF的表达水平。建立荷人骨肉瘤MG63裸小鼠动物模型，以Ad-VEGF—siRNA瘤组织注射治疗，检测移植瘤组织中VEGF的表达情况及Ad．VEGF—siRNA对肿瘤生长和肺转移的抑制作用。结果：成功构建了表达VEGF—siRNA的重组腺病毒载体Ad—VEGF-siRNA；体外与体内实验检测Ad—VEGF—siRNA转染骨肉瘤细胞MG63后显著抑制VEGF表达。荷人骨肉瘤裸鼠经Ad—VEGF—siRNA治疗后，显示其对移植骨肉瘤生长有明显抑制作用（P〈0．05），并且对骨肉瘤的肺转移有显著的抑制作用（P〈0．05）。结论：所构建的Ad—VEGF—siRNA可以有效抑制骨肉瘤中VEGF表达，使裸鼠移植骨肉瘤生长减慢，并且显著抑制荷瘤裸小鼠肺转移的发生。

英文摘要：

Objective： To investigate the inhibitory effect of adenovirus-mediated VEGF-siRNA on transplanted osteosarcoma in nude mice. Methods： VEGF-siRNA gene was cloned into the genome of replication-deficient adenovirus to construct Ad-VEGF-siRNA; the latter was then used to infect osteosarcoma MC,63 cell line in vitro; and the expression of VEGF gene was detected by RT-PCR. Osteosarcoma transplantation model was established in nude mice; VEGF expression in tumor tissue was analyzed and the inhibitory effect on tumor growth and lung metastasis were also observed. Results： The recombinant adenovirus vector Ad-VEGF- siRNA was successfully constructed. In vivo and in vitro experiment both showed that Ad-VEGF-siRNA significantly downregulated VEGF expression in MC,63 cells and transplanted tumor tissue. It was found that Ad-VEGF-siRNA significantly inhibited transplanted osteosarcoma growth （P 〈 0.05 ）and lung metastasis （P 〈 0.05 ）in tumor-bearing nude mice. Conclusion： The constructed Ad-VEGF-siRNA can effectively inhibit the expression of VEGF, depress transplanted tumor growth, and inhibit lung metastasis in nude mice.