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中文摘要：
在pH4．5的0．2mol／L Britton-Robinson（B-R）缓冲溶液中，茜素兰S（ABS）于-0．51V（vs．SCE）产生一个灵敏的线性扫描二阶导数极谱还原峰。当在上述溶液中加入DNA后，茜素兰S在-0．51V处还原峰的峰电流降低并且峰电位正移，说明两者之间可以通过嵌插作用而结合。结合反应的发生使溶液中游离的茜素兰S浓度降低，相应的还原峰电流降低。实验优化了结合反应条件和电化学测定条件。在最佳条件下，峰电流的降低值同DNA的浓度在1．0～60．0mg／L范围内呈线性关系，线性回归方程为△ip″（nA）=18．13c（mg／L）～7．61（n=9，r=0．998）。方法应用于合成样品中DNA的测定，结果令人满意。
英文摘要：
In pH 4.5 Britton-Robinson （B-R） buffer solution, alizarine blue S （ABS） has a well-defined linear sweep voltammetric wave at -0. 51 V （vs. SCE） on the mercury working electrode. By the addition of DNA into the ABS solution, the reduction peak current of ABS decreases with the positive movement of the peak potential and without the appearance of new peaks, which indicates that there is intercalation between ABS and DNA. The optimal conditions of reaction and the electrochemical detection were selected. Under the optimal conditions, there is little interferences from most metal ions and amino acids. The calibration graph for fsDNA is linear in the range of 1.0-60.0 mg/L with the linear regression equation of △ip″（nA）= 18. 13c（mg/L） - 7. 61（n=9, r=0. 998）,and the detection limit of 0. 82 mg/L. The analytical results of synthetic samples are satisfactory with the relative standard deviations of five parallel determination of 1.94% -3.45 %.
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