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中文摘要：
应用极谱络合吸附波方法检测了草丁膦乙酰转移酶基因（PAT基因）的DNA特定序列。用水热法合成表面具有自由羧基的硒化铅（PbSe）纳米粒子，以乙基-（3-二甲基丙基）碳二亚胺盐酸盐（EDC）为偶联活化剂，将其标记于合成的5’端氨基修饰的寡聚核苷酸片段上，制成DNA探针，用于检测互补的目标DNA序列。以十六烷基三甲基溴化铵（CTAB）阳离子表面活性剂修饰的碳糊电极作为基底电极，通过静电作用将来自于PAT基因的目标DNA特定序列固定在电极表面，并与标记有PbSe纳米粒子的互补DNA探针杂交。以铅-铜铁试剂-六次甲基四胺-HAc极谱络合吸附波测定氧化溶解Pb—Se得到的Pb^2＋，成功检测了PAT基因的DNA特定序列。测定目标DNA的线性范围为1．0×10^-11至1．0×10^-2 mol·L^-1，检测限为8．7×10^-12 mol·L^-1（3σ）。此方法测定目标DNA序列检测限低，灵敏度高，线性范围宽，重现性好，操作简捷方便，对互补和非互补序列具有很好的识别能力。
英文摘要：
The polarographic complex adsorptive wave was firstly used to detect DNA specific sequences of the phosphinothricin acetyltransferase（PAT）gene. Lead selenide （PbSe） nanoparticle surface-modified by free carboxyl was hydrothermally synthesized, and used as a marker to label the DNA probe for identifying complementary target DNA sequence. Sequence-specific DNA related to the PAT gene was used as target DNA sequence and immobilized electrostatically on cetyltrimethylammonium bromide （CTAB） modified carbon paste electrode （CTAB/CPE）, and further hybridized with the complementary probe DNA labeled with PbSe nanoparticles. The hybridization events were monitored by the oxidation dissolution of lead selenide anchored on the hybrids and Pb- cupferron-methenamine-acetic acid （HAe） polarographic complex adsorptive wave. DNA specific sequences related to the PAT gene was determined with a detection range from 1.0×10^-11 to 1.0×10^-2 mol·L^-1, and a detection limit of 8.7×10^-12 mol·L^-1（3σ）. The proposed method showed a good distinguishable ability to the noneomplemen-tary DNA sequences with the complementary DNA sequences.
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