中文摘要：

本研究采用Lipofectamine^TM2000将真核重组表达质粒pVAXI-Vp4染至MA-104细胞中，得到了表达。以荧光抗体检测法和RT-PcR法双重检测了真核重组表达质粒pVAXI-Vp4在MA-104细胞中的表达情况。并将pVAXI-Vp4免疫BALB／c鼠，检测其血清抗体和脾脏中的淋巴细胞增殖情况及CD4^＋，CD8^＋T细胞的数量变化情况。结果表明，猪A组轮状病毒Vp4全基因不但在哺乳动物细胞中获得了表达，而且将真核重组表达质粒pVAXI-Vp4给动物免疫后，获得了免疫效果。这为pVAXI-Vp4 DNA核酸疫苗的进一步推广应用提供了科学依据。

英文摘要：

In this study, the eukaryotic expression recombinant plasmid of pVAXI-Vp4 was transfected into MA104 cells with Lipofectamine^TM2000 and the Vp4 gene was expressed and detected with immunofluorescence and RT-PCR, which indicated that the Vp4 gene was expressed in MA-104 cells. To explore the Vp4 protein protective efficacy, the BALB/c mice was immunied with the recombinant plasmid of pVAXI-Vp4, The protective efficacy of recombinant plasmid of pVAXI-Vp4 was detected by the serum antibody titer, lymphocyte cells content and CD4^＋, CD8^＋ T cells determination. The result showed that the protective efficacy of mice vaccined with recombinant plasmid of pVAXI-Vp4 was better than the control mice vaccined with pVAXI plasmid and saline. This laid a solid foundation for the development of pVAXI-Vp4 DNA vaccine.