中文摘要：

为获得重组鸡α-干扰素并对其进行生物活性研究,本试验对重组大肠杆菌进行诱导表达,收集重组鸡α-干扰素包涵体后进行复性纯化及免疫印迹试验,并通过测定病毒EID50,在接种H9亚型禽流感病毒、新城疫病毒和传染性支气管炎病毒的鸡胚中进行干扰素抗病毒活性试验,运用微变量细胞病变抑制法进行重组鸡α-干扰素抗病毒效价的测定。结果显示复性后重组鸡α-干扰素的免疫印迹试验成功获得了预期条带,在鸡胚中重组鸡α-干扰素具有显著的抗H9亚型禽流感病毒、新城疫病毒和传染性支气管炎病毒等活性作用。在CEF/VSV细胞系上测定重组鸡α-干扰素的抗病毒效价为1.5×2^10 U/mg左右,高浓度重组鸡α-干扰素具有一定的细胞毒性。结果表明重组鸡α-干扰素蛋白具有免疫原性和抗原性,在鸡胚内具有较好的抑制或杀灭H9亚型禽流感病毒、新城疫病毒和传染性支气管炎病毒的效果,呈广谱性,抗病毒效价较高,为下一步抗病毒制品的创制奠定基础。

英文摘要：

In order to obtain the recombinant chicken interferon-a （ChIFN-α） and study its biological activity, the recombinant E. coli was induced to express ChIFN-α. After ChIFN-α inclusions were collected for renaturation and purification, Western blotting test for the recombinant ChIFN-α was done. By measuring the EIDso of chicken embryos infected with avian influenza virus （AIV） H9 subtype, Newcastle disease virus （NDV） and infectious bronchitis virus （mV）, we investigated the antiviral activity of the recombinant ChIFN-α and determined its antiviral potency by cytopathic effect inhibition assay. The specific bands were found as expected in Western blotting test of renaturated ChIFN-α. In addition, the ChIFN-α showed significant antiviral activity against AIV H9, NDV and IBV, and antiviral potency of the interferon was about 1. 5×2^10 U according to the test on CEF /VSV cell line. High-concentration ChIFN-α showed cytotoxicity. The recombinant ChIFN-α had immunogenicity and antigenicity. And it could inhibit or kill viruses in chicken embryos including AIV H9, NDV and IBV, which showed broad spectrum and high antiviral activity. The study would promote our research and development of antiviral products in the future.