大豆(Glycine max)分枝在个体和群体水平上均与大豆产量关系密切,因此大豆分枝相关基因的发掘及利用对大豆高产分子育种具有重要意义。文章通过GO(Gene ontology)分类和文献检索共获得植物分枝发育相关基因183个。基于序列相似和结构域相同的原则,从大豆基因组中发掘出大豆分枝相关的候选基因406个。通过收集已发表的大豆分枝相关QTL,利用BioMercator2.1软件,将符合映射条件的35个QTL映射到公共图谱的12个染色体。通过共定位分析发现,在20个分枝相关的QTL区间内存在大豆分枝相关候选基因57个。本文发掘的分枝发育相关基因信息为大豆分枝相关QTL的精细定位和克隆以及大豆分枝发育的分子生物学基础研究提供了参考。
Branch number of soybean is one of agronomic traits that closely related with yield in both individual and population levels;therefore,discovering genes related to branching is important for molecular breeding in soybean.In this study,183 genes related to plant branch development had been collected through GO classification and literatures.Based on the principle of similar sequences and conserved domains,406 genes possibly related to branching had been identified at the whole genome level in soybean.On the other hand,integration of published QTLs related to branching was carried out using BioMercator2.1,and a total of 35 QTLs was located on 12 chromosomes.Co-localization analysis of QTLs and genes related to branching suggested that 57 genes were located in 20 intergrated QTL regions.These results provided useful information for fine mapping of QTLs related to branching and molecular biology study of branch development in soybean.