中文摘要：

Na ⁺/H⁺ 反脚夫很好被记录了由调整细胞的离子动态平衡提高工厂盐忍耐。这里，从大豆的通常认为的 Na ⁺/H⁺ 反脚夫基因相当或相同的事物 GmNHX2 被克隆并且预言了与 10 个通常认为的 transmembrane 领域编码 534 氨基酸的蛋白质。GmNHX2 在所有大豆工厂纸巾被表示，但是在根和它的表示充实被 NaCl 和聚乙烯乙二醇(木钉) 治疗导致。GmNHX2 比 AtNHX1 和 AtSOS1 的与 LeNHX2 和 AtNHX6 展出更大的顺序类似。尽管种系发生的分析与 organellar (tonoplast 和泡) 聚类 GmNHX2 反脚夫， GmNHX2-EGFP (提高的绿荧光灯的蛋白质) 熔化蛋白质在血浆膜或转基因的植物房间的细胞器膜是可能局部性的。而且，当时，表示 GmNHX2 的转基因的 Arabidopsis 植物在萌芽和幼苗阶段期间对高 NaCl 集中更容忍与野类型的植物相比。这些结果建议 GmNHX2 是一个膜 Na ⁺/H⁺ 反脚夫并且可以工作在盐应力下面调整离子动态平衡。

英文摘要：

Na＋/H＋ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na＋/H＋ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCI and polyethylene glycol （PEG） treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar （tonoplast and vesicles） antiporters, the GmNHX2-EGFP （enhanced green fluorescent protein） fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells, Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na＋/H＋ antiporter and may function to regulate ion homeostasis under salt stress.