中文摘要：

目的建立同时测定款冬花药材中12种成分的超高效液相色谱（UPLC）方法,并对不同产地药材的质量状况进行评价。方法采用BEH C18（100 mm×2.1 mm,1.7μm）色谱柱;流动相为乙腈（A）-0.03%三氟乙酸水（B）,梯度洗脱,体积流量0.5 m L/min,检测波长254 nm,柱温40℃。通过计算不同成分的变异系数,采用聚类分析（HCA）和主成分分析（PCA）对不同产地的款冬花药材进行分析。结果建立的测定方法符合方法学要求,款冬花药材中不同成分的变异系数差别较大,其中金丝桃苷的变异系数达到0.91。PCA和HCA显示野生款冬和栽培款冬具有明显差异,其中野生款冬中绿原酸、咖啡酸、芦丁、金丝桃苷、异绿原酸B、异绿原酸A、异绿原酸C、款冬酮等成分量相对较高。结论不同产地款冬花药材存在较大差异,体现在不同成分的变异范围明显不同,野生和栽培药材存在差异,为保证临床用药的安全有效,款冬花药材间的差异性对临床疗效的影响有待进一步深入研究。

英文摘要：

Objective To establish a UPLC method for simultaneously determining 12 components and evaluating the quality of Farfarae Flos from various origins.Methods The UPLC method was achieved by BEH C_（18） column（100 mm × 2.1 mm,1.7 μm） using a mobile phase made up of acetonitrile（A）-0.03%trifluoroacetic acid in water（B）.The flow rate and detection wavelength were 0.5 mL/min and254 nm,respectively.The column temperature was 40℃.Coefficients of variation（CV） were calculated,then PCA and HCA were applied to analyzing Farfarae Flos from different origins.Results The method for content determination was in agreement with methodological requirements.The results showed that CV values of different components differed greatly,and the hyperoside showed the largest CV value at 0.91.PCA and HCA analysis revealed the wild and cultivated Farfarae Flos were different,and the wild samples contained more chlorogenic acid,caffeic acid,rutin,hyperoside,3,4-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,4,5-dicaffeoylquinic,and tussilagone.Conclusion There exist the significant differences among Farfarae Flos from various origins,and their CV values are also different.In addition,the wild samples also differ from those of the cultivated ones.To guarantee the safety and effectiveness in clinical,the impact of chemical differences of different Farfarae Flos on the clinical efficacy should be further studied.