中文摘要：

目的观察补肾通脉方对伴胰岛素抵抗（insulin resistance,IR）多囊卵巢综合征（polycystic ovarian syn-drome,PCOS）大鼠胰岛素靶器官中胰岛素受体底物-1（insulin receptor substrate-1,IRS-1）表达的影响,探讨补肾通脉方改善伴IR的PCOS的机制。方法23日龄雌性SD大鼠皮下注射硫酸普拉睾酮钠9 mg/（100 g.d）共20 d,联合高脂饮食喂养80 d建立IR的PCOS大鼠模型。成模大鼠随机分为模型组（27只）和中药组（23只）,另设13只正常组。中药组以补肾通脉方干预。各组大鼠动情后期于门静脉推注胰岛素前后各处死一部分。逆转录聚合酶链式反应（RT-PCR）检测各组大鼠肝脏、脂肪组织IRS-1 mRNA表达,免疫印迹法（Western blot）检测各组大鼠肝脏、脂肪组织中IRS-1蛋白表达,免疫组化检测卵巢组织IRS-1蛋白表达。结果模型组IRS-1在肝脏、脂肪组织中mRNA及蛋白表达,以及卵巢中蛋白表达,均明显低于正常组（均P〈0.05）,中药组均显著高于模型组（均P〈0.05）。各组IRS-1的表达在胰岛素刺激后均较刺激前明显增多（均P〈0.05）。结论补肾通脉方可提高伴IR的PCOS大鼠肝脏、脂肪和卵巢中IRS-1的表达,这可能是其改善IR和促进排卵的机制之一。

英文摘要：

Objective To investigate the effects of Bushen Tongmai recipe（BSTMR） on the expression of insulin receptor substrate-1（IRS-1） in insulin target-tissues of polycystic ovarian syndrome（PCOS） rats with insulin resistance（IR）.Methods Twenty-three-day-old female SD rats were injected subcutaneously with sodium prasterone sulfate [9 mg/（100 g·d）] for 20 days,and fed on high-fat forage for 80 days to induce rat model of PCOS with IR.Then the insulin-resistant PCOS rats were randomly divided into the model group（27 rats） and the treated group（23 rats）.Meanwhile,a group of 13 rats of the same age was considered as the normal group.The treated group was administered with BSTMR.A portion of each group was given insulin injection through portal vein in late estrus,whereas rest of each group didn＇t receive insulin injection.The mRNA and protein expression levels of IRS-1 in liver and fatty tissues were detected by RT-PCR and Western blot,respectively.The protein levels of IRS-1 in ovary tissues were assayed by immunohistochemistry.Results As compared with the normal group,the expression of IRS-1 mRNA and protein in liver,fatty tissues and the protein expression of IRS-1 in ovary in the model group were decreased markedly（P0.05）.As compared with the model group,those criteria in the treated group were increased significantly（P0.05）.After insulin stimulation,the expression of IRS-1 mRNA and protein in liver and fatty tissues,and the protein expression of IRS-1 in ovary in three groups were all obviously higher than those before insulin stimulation（P0.05）.Conclusion BSTMR could elevate the expression of IRS-1,which may be one of the mechanisms of BSTMR improving insulin signal transduction and promoting ovulation in PCOS rats with IR.