中文摘要：

观察 Bushen Tongmai 配方的效果的目的(琛ヨ偩閫氳剦鏂？BSTMR ) 在 mRNA 和蛋白质 kinase B alpha 的蛋白质表情上(在 polycystic 的肝、脂肪质、肌肉发达、卵巢的纸巾的 PKB 伪) 有胰岛素抵抗(红外) 并且到的卵巢(PCO ) 老鼠在对待红外和排卵机能障碍探索 BSTMR 的可能的分子的机制。方法女性 22-day-old SD 老鼠与钠 prasterone 硫酸盐皮下地被注射(9 mg 路 1 00g ? 1 路 d ? 1 ) 为 20 天并且用高脂肪的饮食喂了 80 天与红外导致 PCO 老鼠。然后， PCO 老鼠随机被划分成模型组(n=23 ) 和对待的组(n=21 ) 。对待的组与 BSTMR 被管理 2 个星期。同时，有一样的年龄的 15 只老鼠的一个组被用作控制组。在卵巢的组织学的变化被检验。Fasting 血葡萄糖(FBG ) 被葡萄糖 oxidase 方法决定。浆液 fasting 胰岛素(鳍) 被放射性免疫测定(RIA ) 决定。PKB 伪的 mRNA 水平被反向的抄写聚合酶链反应(RT-PCR ) 测量。染色的 Immunohistochemistry 和西方的污点分析被采用在目标纸巾检测蛋白质表示。结果与控制组，相比，在模型组的卵巢显示出多重小囊的包囊，在模型组的 FBG 和鳍的层次显著地增加了( P < 0.05 或 P < 0.01 ，分别地)，并且胰岛素敏感索引( ISI )显然减少了( P < 0.01 )。在在模型组的目标纸巾的 PKB 伪的 mRNA 和蛋白质表达式是比在控制组的那些戏剧性地低的(P < 0.05 或 P < 0.01 ) 。与模型组相比，在对待的组的卵巢的滤泡的阶层 granulosum 显著地增加了，在对待的组的鳍的水平显然减少了( P < 0.01 )，在对待的组的 ISI 显著地改善了( P < 0.01 )，并且在对待的老鼠的目标纸巾的 PKB 伪 的 mRNA 和蛋白质表情显著地被提高( P < 0.05 或 P < 0.01 )。结论 BSTMR 能与红外在 PCO 老鼠改进红外和排卵机能障碍，并且它的分子的机制可能与 mRNA 的举起和在有红外的 PCO 老鼠的目标纸巾的 PKB 伪的蛋白质表情是密切相关的。关键

英文摘要：

Objective： To observe the effects of Bushen Tongmai Recipe （补肾通脉方, BSTMR） on mRNA and protein expressions of protein kinase B alpha （PKB α） in hepatic, adipose, muscular, and ovarian tissues of polycystic ovary（PCO） rats with insulin resistance （IR） and to explore the possible molecular mechanism of BSTMR in treating IR and ovulation dysfunction. Methods： Female 22-day-old SD rats were injected subcutaneously with sodium prasterone sulfate （9 mg.100g^-1.d^-1） for 20 days and fed with high-fat diet for 80 days to induce PCO rats with IR. Then, the PCO rats were randomly divided into the model group （n=23） and the treated group （n=21）. The treated group was administered with BSTMR for 2 weeks. Meanwhile, a group with 15 rats of the same age was used as the control group. The histological changes in the ovaries were examined. Fasting blood glucose （FBG） was determined by the glucose oxidase method. Serum fasting insulin （Fins） was determined by radioimmunoassay （RIA）. The mRNA level of PKB ~ was measured by reverse transcription polymerase chain reaction （RT-PCR）. Immunohistochemistry staining and Western blot analysis were employed to detect the protein expression in target tissues. Results： Compared with the control group, the ovaries in the model group showed multiple follicular cysts, levels of FBG and Fins in the model group increased markedly （P〈0.05 or P〈0.01, respectively）, and the insulin sensitive index （ISI） decreased obviously （P〈0.01）. The mRNA and protein expressions of PKB e in target tissues in the model group were dramatically lower than those in the control group （P〈0.05 or P〈0.01）. Compared with the model group, the stratum granulosum of the ovarian follicle in the treated group increased markedly, the level of Fins in the treated group decreased obviously （P〈0.01）, ISI in the treated group improved markedly （P〈0.01）, and the mRNA and protein expressions of PKBα in target tissues of the treated rat