中文摘要：

目的：探讨鸡胚胎干细胞（ESC）和精原干细胞（SSCs）在基因表达水平上的差异。方法：传至二代的ESC和SSCs，采用免疫荧光和碱性磷酸酶检测法联合鉴定其干细胞特性，RT-PCR方法检测两者相关基因的表达差异。结果：两种处于未分化状态时的干细胞基因表达存在差异：未分化的ESC表达基因GDF3基因和Nanog基因；SSCs表达特定基因C-kit、Cvh和Stra$基因。结论：两种干细胞在基因表达水平上有差异，为ESC与SSCs在基因水平上的鉴定提供参考依据。

英文摘要：

Objective：To determine genes differential expression between the chicken embryonic stem cell （ESC） and spermatogonial stem cell （SSCs）. Method：The characteristics of ESC and SSCs were analyzed with the expression of alkaline phosphatase （AKP）, immuno- logic markers and pluripotency markers. Reverse transcription polymerase chain reaction （ RT ＇ PCR） was used to detect the differential expression of genes. Result： The two stem cells all expressed AKP and stage - specific embryonic antigen - 1 （ SSEA - 1 ）, which presented that the two cell maintained the proliferation in an undifferentiated state. In addition, the ESC expressed growth and differentiation factor 3 （GDF3） and Nanog genes while SSCs expressed c - kit, chicken vasa homolog （Cvh） and stimulated by retinoic acid gene 8 （Strag）. Concluslon：There were differential expression of genes between the two stem cells, which can provide a basis for identifying the two cells.