中文摘要：

研究了不同浓度壬基酚（nonylphen01）对莱茵衣藻（Chlamydomonasreinhardtii）生长、色素含量、抗氧化酶活性和脂质过氧化作用的影响。结果表明，随着P（壬基酚）（0～0．8mg·L^-1）的升高，莱茵衣藻的生长受到抑制，培养5d时0．8mg·L^-1壬基酚处理组抑制率达到84．4％。叶绿素a含量随着壬基酚浓度的升高而呈先升高后降低的变化趋势，类胡萝卜素含量则逐渐降低，0．6mg·L^-1壬基酚处理组叶绿素a和类胡萝卜素含量分别比对照组下降16．7％和37．2％（P〈0．05）；总可溶性蛋白和丙二醛（MDA）含量随壬基酚浓度的升高而呈下降趋势，0．4和0．6mg·L^-1壬基酚处理组这两个指标与对照组间差异均达显著水平（P〈0．05）。超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性随壬基酚浓度的增加而不断上升，至P（壬基酚）为0．6mg·L^-1时SOD和CAT活性极显著升高（P〈0．001），分别为对照组的25．0倍和32．1倍。还原型谷胱甘肽（GSH）含量在一定浓度范围（0—0．2mg·L^-1）内随壬基酚浓度的升高而升高，当P（壬基酚）〉0．2mg·L^-1时则下降，表明壬基酚胁迫可诱导GSH含量增加。

英文摘要：

Effects of nony｜phenol （NP） stress on growth, pigment content, activity of antioxidant enzymes and lipid per- oxidation of Chlamydomonas reinhardtii were studied. Results show that the growth of C. reinhardtii was inhibited by in- creasing NP concentration in the range of 0 -0. 8 mg·L^-1 and after 5 days of incubation the inhibition rate in Treatment 0. 8 mg·L^-1 NP reached 84.4%. The content of chlorophyll a increased first and then decreased but carotenoid content decreased gradually with rising NP concentration. In Treatment 0. 6 mg ·L^-1 NP the content of chlorophyll a and the content of carotenoid was 16. 7% and 37.2% , respectively, lower than that in the control（P 〈0. 05）. The content of total soluble protein and malondialdehyde （MDA） decreased with increasing NP concentration, and was significantly lower in Treatment 0.4 and 0. 6 mg·L^-1 NP than in the control（ P 〈 0. 05 ）. Superoxide dismutase （SOD） and catalase （CAT） activity increased with increasing NP concentration and was 25.0 and 32. 1 times higher in Treatment 0. 6 mg ·L^-1 NP than in the control（P 〈 0. 001 ）, respectively. The content of glutathione （GSH） also increased with increasing NP con- centration in the range of 0 - 0. 2 mg ·L^-1 and began to turn downwards when NP concentration rose beyond 0. 2 mg·L^-1, indicating that NP stress may induce rise of GSH content.