中文摘要：

目的 探讨阿托伐他汀对人脐静脉内皮细胞增殖和内皮脂酶mRNA表达的影响。方法 不同浓度阿托伐他汀（2、4、6、8及10μmol／L）与人脐静脉内皮细胞分别孵育2、4、8、12及24h后，用半定量逆转录聚合酶链反应法检测人脐静脉内皮细胞内皮脂酶mRNA的表达，用嗜银蛋白分析法观察人脐静脉内皮细胞的增殖情况。结果 阿托伐他汀抑制人脐静脉内皮细胞内皮脂酶mRNA表达，该作用呈剂量依赖性和时间依赖性。阿托伐他汀作用下，人脐静脉内皮细胞内嗜银蛋白颗粒随浓度的增加和作用时间的延长减少趋势越明显。两因素直线相关分析显示，内皮脂酶mRNA表达与嗜银蛋白颗粒数呈正相关关系（r=0．963，P〈0．01）。结论 阿托伐他汀抑制人脐静脉内皮细胞的增殖，抑制人脐静脉内皮细胞内皮脂酶mRNA的表达，且呈时间一剂量依赖性。人脐静脉内皮细胞的增殖与人脐静脉内皮细胞内皮脂酶mRNA的表达呈正相关。

英文摘要：

Aim To evaluate the effect of atorvastatin on proliferation and mRNA expression of endothelial lipase （EL） in cultured human umbilical vein endothelial cells （ hUVEC ）. Methods Each group of cultured hUVEC was incttbated with atorvastatin of 2, 4, 6, 8and 10μmol/L for2, 4, 8, 12 and24h separately. The expression Of ELmRNAinhUVEC was detected by quantitative competitive reverse transcription-polymerase chain reaction （ RT-PCR）. The proliferation of hUVEC was observed by AgNORs analytical method. Results Atorvastatin markedly restrained the expression of EL mRNA in hUVEC. The effect appeared dose dependence and time dependence. With the effect of atorvastatin, the quantity of AgNORs were decreased. The downtrend was evident along with the increased concentration and the prolonged time. There was positive correlativity between the expression of EL mRNA and the quantity of AgNORs （ r = 0. 963, P 〈 0.01 ）. Conclusions Atorvastatin dose-dependently and time-dependently restrained the proliferation of hUVEC. As a result, the expression of EL mRNA was decreased too. There was a positive correlativity between them.