目的在不同海拔进行当归Angelica sinensis生态适应性实验,探索影响当归阿魏酸积累的关键因子。方法通过田间实验测定当归阿魏酸量的变化和生理生化指标、光合参数、生态因子。结果当归根中阿魏酸量随海拔升高而增加,且海拔2780m处理比海拔2360m处理高14.5%,差异显著(P〈0.05)。分析影响当归阿魏酸积累的关键因子表明,降雨量(r=0.8988)和温度(r=-0.7991)是关键生态因子,可溶性糖(r=-0.9749)和超氧化物歧化酶(SOD)(r=-0.8408)是关键生理生化因子,湿度(r=0.9699)和光合活性辐射(r=0.9467)是关键光合参数因子。结论适当升高种植海拔,增加降雨量和湿度,降低温度和可溶性糖量均有利于当归中阿魏酸的转化积累。
Objective The ecological adaptability of Angelica sinensis was studied at different altitudes to explore the key factors on the accumulation of ferulic acid (FA) in A. sinensis. Methods Using the field test, the changes of physiological and biochemical indexes, photosynthetic parameters, ecological factors, and FA content were determined. Results FA content in the roots of A. sinensis was increased with the altitude rising, and the FA content in A. sinensis from 2 780 m was higher than that from 2 360 m by 14.5% (P 〈 0.05). The key factors that affected the accumulation of FA were analyzed; Rainfall (r = 0.898 8) and temperature (r = -0.799 1) were the key ecological factors; Soluble sugar (r = -0.974 9) and superoxide dismutase (SOD) (r = -0.840 8) were the key physiological and biochemical factors; Relative humidity (r = 0.969 9) and photosynthetically active radiation (PAR) (r = 0.946 7) were the key photosynthetic factors. Conclusion Properly rising the altitude of planting, increasing the rainfall and humidity, and decreasing the temperature and soluble sugar are benefitial to the transformation and accumulation of FA in A. sinensis.