目的研究甘肃岷县不同海拔对当归渗透调节物质的影响,为当归生态适宜区选择提供参考。方法采用田间试验和实验室实验相结合的方法研究不同海拔当归体内丙二醛(MDA)、脯氨酸(Pro)和可溶性糖(SS)含量变化,从细胞渗透调节方向探索当归适宜种植海拔。结果随海拔升高,MDA,Pro含量先降低后增加,而SS含量保持降低趋势,海拔间差异显著。其中MDA含量在海拔2 570 m和2 780 m分别显著低于海拔2 360 m的52.4%和38.1%,海拔2 570 m显著低于海拔2 780 m的23.1%;Pro含量在海拔2360 m和海拔2 780 m分别显著高于海拔2 570 m的12.4%和13.6%;SS含量在海拔2 360 m显著高于海拔2 780 m的17.9%,而与海拔2 570 m间差异不显著。结论海拔升高可降低当归体内MDA,Pro和SS含量,利于细胞膜稳定,故从当归细胞渗透调节角度分析,在2 300~2 800 m范围内可提升当归种植海拔,扩大当归生态区域,降低连作障碍。
Objective For expanding ecological planting region of Angelic sinensis,the effect of altitude to osmotic regulation substances was studed. Methods Using field experiment and laboratory experiment to study the difference of Malondialdehyde( MDA),Free proline( Pro) and Soluble sugar( SS) content about three different altitudes,the aim was to exploring the appropriate altitude from the angle of osmotic regulation. Results With the elevations rising,the MDA,Pro content were reduced first and increased follow,but SS contents were reduced. MDA content at altitude of 2 570 m and 2 780 m were significantly lower than 2 360 m by 52. 4% and 38. 1%,altitude of2 570 m was significantly lower than altitude of 2 780 m by 23. 1%; Pro contents at altitude of 2 360 m and 2 780 m were significantly higher than altitude of 2 570 m by 12. 4% and 13. 6%; SS contents at altitude of 2 360 m were significantly higher than altitude of 2 780 m by 17. 9%,but no significant difference with altitude of 2 570 m. Conclusion Rising elevation can reduce MDA,Pro and SS content of Angelica sinensis,which is beneficial to the cell membrane stability. So from the angle of osmotic adjustment to analysis,that is feasilble to promote planting altitude and expand ecological area in the range of 2 300 m to 2 800 m.